Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   NML25_RS14300 Genome accession   NZ_CP101222
Coordinates   2965187..2966614 (+) Length   475 a.a.
NCBI ID   WP_000980560.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-144     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2960187..2971614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML25_RS14260 (NML25_14260) yeeW 2960371..2960565 (+) 195 WP_000988600.1 DUF5983 family protein -
  NML25_RS14265 (NML25_14265) - 2960611..2960691 (+) 81 Protein_2796 hypothetical protein -
  NML25_RS14270 (NML25_14270) - 2960980..2961087 (-) 108 WP_074147515.1 transposase domain-containing protein -
  NML25_RS27445 - 2961039..2961362 (+) 324 WP_269447326.1 EutP/PduV family microcompartment system protein -
  NML25_RS14280 (NML25_14280) tmaR 2961463..2961792 (-) 330 WP_000450409.1 DUF496 family protein -
  NML25_RS14285 (NML25_14285) yeeA 2961964..2963022 (-) 1059 WP_001200888.1 FUSC family protein -
  NML25_RS14290 (NML25_14290) sbmC 2963220..2963693 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  NML25_RS14295 (NML25_14295) dacD 2963812..2964978 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  NML25_RS14300 (NML25_14300) sbcB 2965187..2966614 (+) 1428 WP_000980560.1 exodeoxyribonuclease I Machinery gene
  NML25_RS14305 (NML25_14305) tsuB 2966657..2966884 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  NML25_RS14310 (NML25_14310) tsuA 2966898..2967956 (-) 1059 WP_000492349.1 thiosulfate utilization transporter TsuA/YeeE -
  NML25_RS14315 (NML25_14315) plaP 2968135..2969493 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  NML25_RS14320 (NML25_14320) yoeI 2969483..2969545 (-) 63 WP_010723108.1 membrane protein YoeI -
  NML25_RS14325 (NML25_14325) yeeY 2969760..2970689 (-) 930 WP_032180084.1 LysR substrate-binding domain-containing protein -
  NML25_RS14330 (NML25_14330) yeeZ 2970735..2971559 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54392.67 Da        Isoelectric Point: 5.3498

>NTDB_id=609255 NML25_RS14300 WP_000980560.1 2965187..2966614(+) (sbcB) [Escherichia coli strain EC21Z-144]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
ERLGIARQHCLDNLKVLRENPQVRDKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDDNEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQLYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=609255 NML25_RS14300 WP_000980560.1 2965187..2966614(+) (sbcB) [Escherichia coli strain EC21Z-144]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGATGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCACCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGACGCT
GAGCGTTTAGGGATTGCTCGTCAACACTGCCTGGATAACCTGAAAGTGTTGCGCGAAAACCCGCAAGTACGCGACAAAGT
TGTGGCAATTTTTGCAGAAGCTGAACCGTTTACACCATCAGATAATGTAGATGCACAGCTTTATAATGGCTTTTTTAGCG
ACGCCGATCGTGCAGCAATGAAAATCGTGCTGGAGACTGAACCGCGTAATTTACCGGCTCTGGATATCACCTTTGTTGAT
AAGCGTATCGAGAAACTCCTGTTCAACTACCGCGCCCGCAACTTCCCCGGCACGTTAGATGACAACGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCTGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583