Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML26_RS20100 Genome accession   NZ_CP101210
Coordinates   4104242..4104682 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain EC21Z-147     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4099242..4109682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML26_RS20080 (NML26_20090) aroP 4100243..4101613 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  NML26_RS20085 (NML26_20095) ampE 4101656..4102510 (-) 855 WP_000171975.1 beta-lactamase regulator AmpE -
  NML26_RS20090 (NML26_20100) ampD 4102507..4103058 (-) 552 WP_000923730.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NML26_RS20095 (NML26_20105) nadC 4103146..4104039 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NML26_RS20100 (NML26_20110) pilA 4104242..4104682 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NML26_RS20105 (NML26_20115) pilB 4104692..4106077 (+) 1386 WP_001025165.1 type II secretion system protein GspE Machinery gene
  NML26_RS20110 (NML26_20120) hofC 4106067..4107269 (+) 1203 WP_000157293.1 protein transport protein HofC -
  NML26_RS20115 (NML26_20125) guaC 4107304..4108347 (-) 1044 WP_001217338.1 GMP reductase -
  NML26_RS20120 (NML26_20130) - 4108503..4108547 (-) 45 WP_120795372.1 protein YacM -
  NML26_RS20125 (NML26_20135) coaE 4108572..4109192 (+) 621 WP_001269512.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=609194 NML26_RS20100 WP_000360904.1 4104242..4104682(+) (pilA) [Escherichia coli strain EC21Z-147]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=609194 NML26_RS20100 WP_000360904.1 4104242..4104682(+) (pilA) [Escherichia coli strain EC21Z-147]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363