Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LCF45_RS25520 Genome accession   NZ_CP084035
Coordinates   4937215..4938564 (-) Length   449 a.a.
NCBI ID   WP_078206576.1    Uniprot ID   -
Organism   Bacillus sp. 41-22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4932215..4943564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCF45_RS25485 (LCF45_25455) - 4932336..4933031 (-) 696 WP_002129924.1 hypothetical protein -
  LCF45_RS25490 (LCF45_25460) - 4933018..4933806 (-) 789 WP_016121052.1 hypothetical protein -
  LCF45_RS25495 (LCF45_25465) - 4933838..4934422 (-) 585 WP_263894360.1 hypothetical protein -
  LCF45_RS25500 (LCF45_25470) - 4934541..4935035 (-) 495 WP_002184661.1 hypothetical protein -
  LCF45_RS25505 (LCF45_25475) hpf 4935324..4935866 (-) 543 WP_002089603.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LCF45_RS25510 (LCF45_25480) cspC 4936187..4936384 (-) 198 WP_001990088.1 cold shock protein CspC -
  LCF45_RS25515 (LCF45_25485) - 4936511..4937215 (-) 705 WP_078177895.1 ComF family protein -
  LCF45_RS25520 (LCF45_25490) comFA 4937215..4938564 (-) 1350 WP_078206576.1 ATP-dependent helicase ComFA Machinery gene
  LCF45_RS25525 (LCF45_25495) - 4938695..4940044 (-) 1350 WP_199626989.1 NlpC/P60 family protein -
  LCF45_RS25530 (LCF45_25500) - 4940278..4940592 (-) 315 WP_002089608.1 winged helix-turn-helix transcriptional regulator -
  LCF45_RS25535 (LCF45_25505) - 4940755..4941597 (-) 843 WP_002016143.1 DegV family protein -
  LCF45_RS25540 (LCF45_25510) - 4941836..4942471 (+) 636 WP_033731072.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50861.27 Da        Isoelectric Point: 9.4434

>NTDB_id=609191 LCF45_RS25520 WP_078206576.1 4937215..4938564(-) (comFA) [Bacillus sp. 41-22]
MLAGRQLLLEELSSDLQNKLDHLKKHREVVCVQGVIKKSSKYMCQRCGNIEQRLFASFLCKRCSEVCAYCRKCITMGRVS
ECAVLVRGIAEIQGESYLNPLQWEGILSDGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLQYAVNEAM
KEKAARIYLTATPDEKWKRKLRSGKQKGVIISGRYHRHPLPVPIFRWCGNWKKGLIHKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQVDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTIKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=609191 LCF45_RS25520 WP_078206576.1 4937215..4938564(-) (comFA) [Bacillus sp. 41-22]
ATGTTAGCTGGTAGACAGTTACTGTTAGAAGAACTCTCTTCAGATTTGCAGAATAAATTGGATCATCTGAAAAAGCACCG
AGAAGTCGTTTGTGTGCAAGGGGTAATTAAGAAGTCTTCAAAATATATGTGTCAGCGCTGCGGAAATATAGAGCAGCGAC
TATTTGCGTCATTTTTATGTAAGAGGTGCAGTGAAGTATGTGCGTATTGCCGTAAGTGTATAACGATGGGAAGGGTCAGT
GAATGTGCTGTACTTGTGCGCGGGATTGCTGAAATACAAGGAGAAAGTTATTTGAATCCGTTACAGTGGGAAGGCATTTT
GTCTGACGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAAAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCGTATCATGCAGATAAGATGTTGCAGTATGCAGTAAACGAAGCGATG
AAAGAGAAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAGTTGCGAAGTGGTAAGCAAAA
AGGGGTTATTATTTCAGGGCGATACCATCGTCATCCATTGCCAGTTCCGATATTTCGGTGGTGCGGAAATTGGAAAAAGG
GTCTTATACATAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAGTTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGGGGAGTAACTATTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAAATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGATTGATAGATTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-409)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

49.66

98.218

0.488
  comFA Latilactobacillus sakei subsp. sakei 23K

40.704

88.641

0.361