Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LCF45_RS20980 Genome accession   NZ_CP084035
Coordinates   4095232..4096275 (-) Length   347 a.a.
NCBI ID   WP_078203155.1    Uniprot ID   -
Organism   Bacillus sp. 41-22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4090232..4101275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCF45_RS20935 (LCF45_20915) - 4090630..4090830 (-) 201 WP_002015009.1 YqzE family protein -
  LCF45_RS20940 (LCF45_20920) - 4090869..4091366 (-) 498 WP_002129082.1 shikimate kinase -
  LCF45_RS20945 (LCF45_20925) - 4091518..4092168 (-) 651 WP_078203154.1 2OG-Fe(II) oxygenase -
  LCF45_RS20950 (LCF45_20930) comGG 4092343..4092714 (-) 372 WP_002129085.1 competence type IV pilus minor pilin ComGG -
  LCF45_RS20955 (LCF45_20935) comGF 4092711..4093172 (-) 462 WP_002129086.1 competence type IV pilus minor pilin ComGF -
  LCF45_RS20960 (LCF45_20940) - 4093156..4093452 (-) 297 WP_002129087.1 hypothetical protein -
  LCF45_RS20965 (LCF45_20945) comGD 4093445..4093900 (-) 456 WP_002167675.1 competence type IV pilus minor pilin ComGD -
  LCF45_RS20970 (LCF45_20950) comGC 4093897..4094196 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  LCF45_RS20975 (LCF45_20955) comGB 4094208..4095245 (-) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  LCF45_RS20980 (LCF45_20960) comGA 4095232..4096275 (-) 1044 WP_078203155.1 competence type IV pilus ATPase ComGA Machinery gene
  LCF45_RS20985 (LCF45_20965) - 4096481..4097176 (+) 696 WP_002014995.1 helix-turn-helix transcriptional regulator -
  LCF45_RS20990 (LCF45_20970) - 4097457..4097699 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  LCF45_RS20995 (LCF45_20975) - 4097807..4099201 (+) 1395 WP_002138338.1 L-cystine transporter -
  LCF45_RS21000 (LCF45_20980) - 4099283..4099675 (-) 393 WP_002190672.1 hypothetical protein -
  LCF45_RS21005 (LCF45_20985) - 4099763..4099978 (-) 216 WP_002067106.1 DUF3912 family protein -
  LCF45_RS21010 (LCF45_20990) - 4100231..4100542 (+) 312 WP_002129099.1 hypothetical protein -
  LCF45_RS21015 (LCF45_20995) - 4100578..4101066 (-) 489 WP_002015119.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39292.79 Da        Isoelectric Point: 9.4642

>NTDB_id=609180 LCF45_RS20980 WP_078203155.1 4095232..4096275(-) (comGA) [Bacillus sp. 41-22]
MNSVELFANMIMKEACRVRASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=609180 LCF45_RS20980 WP_078203155.1 4095232..4096275(-) (comGA) [Bacillus sp. 41-22]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCGAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCACTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGGTTACTCGTATTTACTGGGCCAACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTAGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
AGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

42.955

83.862

0.36