Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   NML27_RS23725 Genome accession   NZ_CP101197
Coordinates   4961201..4963030 (-) Length   609 a.a.
NCBI ID   WP_024186454.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-151     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4956201..4968030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML27_RS23700 (NML27_23695) bioP 4956919..4957818 (-) 900 WP_001551984.1 biotin transporter -
  NML27_RS23705 (NML27_23700) yigL 4957894..4958694 (-) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  NML27_RS23710 (NML27_23705) pldB 4958702..4959724 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  NML27_RS23715 (NML27_23710) rhtB 4959835..4960455 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  NML27_RS23720 (NML27_23715) rhtC 4960517..4961137 (-) 621 WP_000928844.1 threonine export protein RhtC -
  NML27_RS23725 (NML27_23720) recQ 4961201..4963030 (-) 1830 WP_024186454.1 ATP-dependent DNA helicase RecQ Machinery gene
  NML27_RS23730 (NML27_23725) pldA 4963163..4964032 (-) 870 WP_001259693.1 phospholipase A -
  NML27_RS23735 (NML27_23730) yigI 4964197..4964664 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  NML27_RS23740 (NML27_23735) rarD 4964716..4965606 (+) 891 WP_001551980.1 EamA family transporter RarD -
  NML27_RS23745 (NML27_23740) - 4965917..4966765 (+) 849 WP_001551979.1 YPO3983 family protein -
  NML27_RS23750 (NML27_23745) - 4966762..4967253 (+) 492 WP_001551977.1 DUF943 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68377.53 Da        Isoelectric Point: 7.2544

>NTDB_id=609125 NML27_RS23725 WP_024186454.1 4961201..4963030(-) (recQ) [Escherichia coli strain EC21Z-151]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGIGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=609125 NML27_RS23725 WP_024186454.1 4961201..4963030(-) (recQ) [Escherichia coli strain EC21Z-151]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAGATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCTTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAACATTGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCGGATATGGCGTG
GCTGCGTCGTTGTCTGGAAGAGAAACCGCAGGGGCAGTTACAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACCTGTCGCCGTCTGGTGCTGCTAAACTATTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCACGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCCTTCGGCGGCAACTACGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCGACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCATTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389