Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NML27_RS04565 Genome accession   NZ_CP101197
Coordinates   938578..940569 (+) Length   663 a.a.
NCBI ID   WP_000098598.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-151     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 933578..945569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML27_RS04550 (NML27_04545) - 935551..936282 (+) 732 WP_000758911.1 hypothetical protein -
  NML27_RS04555 (NML27_04550) speB 936418..937338 (+) 921 WP_000105562.1 agmatinase -
  NML27_RS04560 (NML27_04555) loiP 937542..938300 (-) 759 WP_001327406.1 metalloprotease LoiP -
  NML27_RS04565 (NML27_04560) recP/tkt 938578..940569 (+) 1992 WP_000098598.1 transketolase Machinery gene
  NML27_RS04570 (NML27_04565) - 940788..941696 (-) 909 WP_000646942.1 prohibitin family protein -
  NML27_RS04575 (NML27_04570) cmtB 942073..942516 (+) 444 WP_001236709.1 PTS mannitol transporter subunit IIA -
  NML27_RS04580 (NML27_04575) cmtA 942544..943932 (+) 1389 WP_000428784.1 PTS mannitol transporter subunit IICB -
  NML27_RS04585 (NML27_04580) yggP 943947..945224 (+) 1278 WP_000853191.1 zinc-binding dehydrogenase -
  NML27_RS04590 (NML27_04585) - 945221..945536 (+) 316 Protein_906 fructose-bisphosphatase class II -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72242.80 Da        Isoelectric Point: 5.5018

>NTDB_id=609072 NML27_RS04565 WP_000098598.1 938578..940569(+) (recP/tkt) [Escherichia coli strain EC21Z-151]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKEFAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQIA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AEQLFEEFGFTVDNVVAKAKALL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=609072 NML27_RS04565 WP_000098598.1 938578..940569(+) (recP/tkt) [Escherichia coli strain EC21Z-151]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCACCCGGAAGTGGGTTACACCGC
AGGTGTAGAAACCACCACCGGTCCGCTGGGTCAGGGTATTGCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATCGTCGACCACTACACCTACGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCGTTCTACGATGACAACGGTAT
TTCTATCGATGGTCACGTTGAAGGCTGGTTTACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGATATCGACGGTCATGACGCGGCATCCATCAAACGCGCAGTAGAAGAAGCGCGCGCCGTGACTGACAAACCGTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACCCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAGCTGGGCTGGAAATACGCGCCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAATTCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCTGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCCAAAGCGAAAGAGTTCATCGCTAA
ACTTCAGGCTAATCCGGCGAAGATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCGTTCGGGCCGCTGTTGCCGG
AATTCCTCGGCGGTTCTGCAGACCTGGCGCCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTACGTGAATTCGGTATGACTGCGATTGCCAACGGTATCTCCCTGCACGGTGGTTT
CCTGCCGTATACCTCAACCTTCCTGATGTTCGTGGAATACGCACGTAATGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGATGGCCCGACTCACCAGCCGGTAGAACAGATCGCT
TCTCTGCGCGTGACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCAGTAGCGTGGAAATACGGCGT
TGAGCGTCAGGACGGCCCGACTGCGCTTATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGTACTGAAGAGCAGCTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGTGCTGGTCAGCCGGAACTGATCTTCATCGCGACCGGTTCTGAA
GTTGAACTGGCTGTTGCCGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTTTCCATGCCGTCTACCGA
CGCATTCGACAAGCAGGACGCGGCTTACCGTGAATCCGTACTGCCGAAAGCGGTTACCGCTCGTGTTGCGGTGGAAGCTG
GCATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGCATGACCACCTTCGGTGAATCTGCTCCG
GCAGAGCAGCTGTTTGAAGAGTTCGGCTTCACCGTTGACAACGTTGTTGCGAAAGCAAAAGCGCTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.176

99.246

0.478