Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NML27_RS01440 Genome accession   NZ_CP101197
Coordinates   325900..327396 (+) Length   498 a.a.
NCBI ID   WP_001551783.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-151     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320900..332396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML27_RS01410 (NML27_01410) tusA 321147..321392 (+) 246 WP_000130615.1 sulfurtransferase TusA -
  NML27_RS01415 (NML27_01415) zntA 321494..323692 (-) 2199 WP_001551784.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NML27_RS01420 (NML27_01420) yhhN 323766..324392 (-) 627 WP_000964718.1 lysoplasmalogenase -
  NML27_RS01425 (NML27_01425) yhhM 324533..324892 (+) 360 WP_000042886.1 DUF2500 domain-containing protein -
  NML27_RS01430 (NML27_01430) yhhL 324895..325164 (-) 270 WP_001295207.1 DUF1145 family protein -
  NML27_RS01435 (NML27_01435) rsmD 325154..325750 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NML27_RS01440 (NML27_01440) pilA 325900..327396 (+) 1497 WP_001551783.1 signal recognition particle-docking protein FtsY Machinery gene
  NML27_RS01445 (NML27_01445) ftsE 327399..328067 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NML27_RS01450 (NML27_01450) ftsX 328060..329118 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NML27_RS01455 (NML27_01455) rpoH 329363..330217 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NML27_RS01460 (NML27_01460) livJ 330488..331591 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NML27_RS01465 (NML27_01465) panM 331791..332174 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54701.53 Da        Isoelectric Point: 4.1660

>NTDB_id=609058 NML27_RS01440 WP_001551783.1 325900..327396(+) (pilA) [Escherichia coli strain EC21Z-151]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPELVAEETPEPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=609058 NML27_RS01440 WP_001551783.1 325900..327396(+) (pilA) [Escherichia coli strain EC21Z-151]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACTGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCGGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCAGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTAGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACTAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATTCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.864

74.096

0.369