Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LCH18_RS02405 Genome accession   NZ_CP083947
Coordinates   500497..501297 (+) Length   266 a.a.
NCBI ID   WP_224468287.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain CSUSB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495497..506297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH18_RS02375 (LCH18_02375) - 495736..496146 (+) 411 WP_224468282.1 hypothetical protein -
  LCH18_RS02380 (LCH18_02380) - 496264..497544 (+) 1281 WP_224468284.1 glycosyltransferase family 4 protein -
  LCH18_RS02385 (LCH18_02385) - 497737..498288 (+) 552 WP_005401706.1 phosphatase PAP2 family protein -
  LCH18_RS02390 (LCH18_02390) iscX 498379..498579 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  LCH18_RS02395 (LCH18_02395) ndk 498690..499121 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  LCH18_RS02400 (LCH18_02400) rlmN 499245..500480 (+) 1236 WP_224468285.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LCH18_RS02405 (LCH18_02405) pilF 500497..501297 (+) 801 WP_224468287.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LCH18_RS02410 (LCH18_02410) - 501288..502064 (+) 777 WP_224468289.1 helix-turn-helix domain-containing protein -
  LCH18_RS02415 (LCH18_02415) ispG 502083..503198 (+) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LCH18_RS02420 (LCH18_02420) hisS 503195..504490 (+) 1296 WP_224468291.1 histidine--tRNA ligase -
  LCH18_RS02425 (LCH18_02425) - 504518..505216 (+) 699 WP_005401704.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30102.10 Da        Isoelectric Point: 8.9758

>NTDB_id=608753 LCH18_RS02405 WP_224468287.1 500497..501297(+) (pilF) [Acinetobacter johnsonii strain CSUSB1]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQRNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMDSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=608753 LCH18_RS02405 WP_224468287.1 500497..501297(+) (pilF) [Acinetobacter johnsonii strain CSUSB1]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCTGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCGAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGATTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

67.91

100

0.684
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647