Detailed information NTG0608658

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: LCH16_RS05505
• Genome accession: NZ_CP083943
• Coordinates: 1096769..1100473 (+)
• Length: 1234 a.a.
• NCBI ID: WP_061890946.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain CSUSB3
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1091769..1105473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCH16_RS05490 (LCH16_05490)	-	1091851..1092360 (-)	510	WP_015417176.1	ferritin-like domain-containing protein	-
LCH16_RS05495 (LCH16_05495)	-	1092429..1093142 (-)	714	WP_079891285.1	DUF421 domain-containing protein	-
LCH16_RS05500 (LCH16_05500)	addB	1093282..1096782 (+)	3501	WP_224463075.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
LCH16_RS05505 (LCH16_05505)	addA	1096769..1100473 (+)	3705	WP_061890946.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
LCH16_RS05510 (LCH16_05510)	sbcD	1100538..1101710 (+)	1173	WP_015417180.1	exonuclease subunit SbcD	-
LCH16_RS05515 (LCH16_05515)	sbcC	1101707..1105099 (+)	3393	WP_224463077.1	exonuclease subunit SbcC	-
LCH16_RS05520 (LCH16_05520)	-	1105115..1105408 (+)	294	WP_060562128.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141154.42 Da
• Isoelectric Point: 5.1266

>NTDB_id=608658 LCH16_RS05505 WP_061890946.1 1096769..1100473(+) (addA) [Bacillus velezensis strain CSUSB3]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKVK
TDYFTRSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEETGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKVGEVDYDEQAALKLGASYPANDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSE
LLEENPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGK
DLLGALRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3705 bp

>NTDB_id=608658 LCH16_RS05505 WP_061890946.1 1096769..1100473(+) (addA) [Bacillus velezensis strain CSUSB3]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACCGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAACTCTCTCTATTAAATCGGGCGAGCATTTC
GACCCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCAGACAGATACACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCCTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAATATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCCTTTAAGCGGGCGAAGGCGGTAAAGG
GCGATGAATTTGACAAAGCGCTTTTGGACGAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAG
ACCGATTACTTTACAAGAAGTCCGCAAGATCACTTAAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGGTTGATGAAGAAGGCCGGCGGGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACGAATCTTGTGCAAGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGACGGGAAATCTGTTTATGGTAGGCGACGTTAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAGTCGGCGAAGTGGATTACGATGA
ACAGGCGGCATTGAAGCTCGGCGCATCATATCCGGCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACTGTGCATTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATTCAGTACCGGGATATCGTGAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGA
CGTCGGGTTACTTTGAAGCCGTGGAGGTAGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGATCGCCCATCGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGCTGACAGGCATGATGACCTGTATCAAAAACTGAGAACCT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGACTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCCCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAA
AGATGCGCAGAGAGCTCTTATCCGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACA
GGACGCCGGGACTTTCCTCATCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGAGAATCCGAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGAGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGAAGCTATTGATATAGAAGAAATTGTCCAGTTTTTCGGAACTGAAATCGGGAAA
GACCTTCTCGGGGCGCTGCGTATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACAGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACTGCGGACGGTCTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.415	100	0.824