Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LCH15_RS00605 Genome accession   NZ_CP083938
Coordinates   135335..136720 (-) Length   461 a.a.
NCBI ID   WP_224649425.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain CSUSB4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 130335..141720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH15_RS00575 (LCH15_00575) - 130367..131131 (+) 765 WP_396121670.1 glycosyltransferase family 25 protein -
  LCH15_RS00580 (LCH15_00580) - 131121..132254 (-) 1134 WP_224649423.1 glycosyltransferase -
  LCH15_RS00585 (LCH15_00585) - 132317..133282 (+) 966 WP_224651238.1 glycosyltransferase family 32 protein -
  LCH15_RS00590 (LCH15_00590) - 133328..133537 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  LCH15_RS00595 (LCH15_00595) - 133680..134618 (-) 939 WP_034382721.1 branched-chain amino acid transaminase -
  LCH15_RS00600 (LCH15_00600) - 134700..135155 (-) 456 WP_224649424.1 glycerate kinase -
  LCH15_RS00605 (LCH15_00605) radA 135335..136720 (-) 1386 WP_224649425.1 DNA repair protein RadA Machinery gene
  LCH15_RS00615 (LCH15_00615) - 137525..137740 (-) 216 WP_156507056.1 hypothetical protein -
  LCH15_RS00620 (LCH15_00620) - 137807..138871 (-) 1065 WP_063662618.1 DUF1963 domain-containing protein -
  LCH15_RS00625 (LCH15_00625) - 139114..140421 (-) 1308 WP_034351570.1 Glu/Leu/Phe/Val dehydrogenase -
  LCH15_RS00630 (LCH15_00630) - 140631..141554 (+) 924 WP_034351313.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48015.36 Da        Isoelectric Point: 7.9367

>NTDB_id=608618 LCH15_RS00605 WP_224649425.1 135335..136720(-) (radA) [Comamonas thiooxydans strain CSUSB4]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSGQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTIAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=608618 LCH15_RS00605 WP_224649425.1 135335..136720(-) (radA) [Comamonas thiooxydans strain CSUSB4]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACACTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAATCGCCTGAGCCAGCCTCAGGGCT
ATGCCGGTCTGGCCAATGCCCAGCCCGTCACCCCGTTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACCCCCAGCGGT
ATCGAGGAGCTTGACCGCGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTGCTGATTGGCGGAGACCCTGGAATAGG
TAAGTCCACACTTCTATTGCAAGCCATGGATGCATTGCATCGTATCGGTCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCGCTGCGCTCGCGCCGGCTCGGGCTGGACAACAGTCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTCGCCACCGTGGAGGCCACCCAGCCCGCCGTGGTGGTCATCGACTCGATCCAGACCGTGTATTCGGGCCA
GCTTTCCTCGGCTCCGGGCTCCGTGGCCCAGGTGCGCGAATGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTCTGGTAGGCCATGTGACCAAGGACGGCGCGCTAGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTATTTCGAAGGCGACACTCACAGTAACTTCCGCCTGGTGCGCGCCATCAAGAACCGCTTTGGTGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGCCAGCACAGCG
AGCCCGTGCCCGGCAGCTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTGGTGGAC
CAGGGCGGCCCGGCTGCGCGTCGCCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCCGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAATGCCGTGGGTGGCGTGCGCATCAACGAGCCGGCAGCCG
ACCTGGCGGTGATGTTGGCAATCACTTCCAGTCTGCGCGGCAAGTCGCTGCCCAAGGGCTTTATCGCGTTTGGCGAGGTC
GGTCTGGCCGGCGAGGTGCGCCCTGCACCGCGTGGCCAGGAGCGGCTCAAGGAGGCGGCAAAGTTGGGATTCACTATCGC
CGTGGTTCCCAAGGCCAATGCTCCCAAGAAGCCCATCGCCGGCCTGACCATTCATGCCGTGGAGCGCGTGGATGAGGCCA
TCAACATCGTGCGCGGCATTGGCTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-226)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus mitis SK321

49.425

94.36

0.466