Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NMD28_RS06645 Genome accession   NZ_CP101001
Coordinates   1367104..1369107 (-) Length   667 a.a.
NCBI ID   WP_000087298.1    Uniprot ID   A7ZPQ7
Organism   Escherichia coli strain ET120     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1362104..1374107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD28_RS06630 (NMD28_06600) aegA 1363217..1365196 (+) 1980 WP_001078870.1 formate-dependent uric acid utilization protein AegA -
  NMD28_RS06635 (NMD28_06605) nudK 1365264..1365839 (+) 576 WP_000193711.1 GDP-mannose pyrophosphatase NudK -
  NMD28_RS06640 (NMD28_06610) ypfG 1365965..1367008 (+) 1044 WP_001270524.1 DUF1176 domain-containing protein -
  NMD28_RS06645 (NMD28_06615) recP/tkt 1367104..1369107 (-) 2004 WP_000087298.1 transketolase Machinery gene
  NMD28_RS06650 (NMD28_06620) tal 1369127..1370077 (-) 951 WP_332152433.1 transaldolase -
  NMD28_RS06655 (NMD28_06625) maeB 1370366..1372645 (+) 2280 WP_000342642.1 NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase -
  NMD28_RS06660 (NMD28_06630) eutS 1372938..1373273 (+) 336 WP_370602673.1 ethanolamine utilization microcompartment protein EutS -
  NMD28_RS06665 (NMD28_06635) eutP 1373286..1373765 (+) 480 WP_000820764.1 ethanolamine utilization acetate kinase EutP -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73041.78 Da        Isoelectric Point: 6.3824

>NTDB_id=608499 NMD28_RS06645 WP_000087298.1 1367104..1369107(-) (recP/tkt) [Escherichia coli strain ET120]
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=608499 NMD28_RS06645 WP_000087298.1 1367104..1369107(-) (recP/tkt) [Escherichia coli strain ET120]
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAATCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGG
CGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCGGAACGTACACTGGCGG
CGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATG
AAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGA
AGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAAAGATTTATCACGCCTGGG
ATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTG
GCAGAAGAGTTTACCCGACGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
ACAGGCAAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCGATGCTGCCTGAGT
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCAGCG
GGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCCTGGGCGAAGATGGTCCGACGCACCAGGCTGTTGAGCAACTGGCCAGC
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCGGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATATTATTCTGATTGCCACCGGTTCAGAGATG
GAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTA
TTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGC
CTGA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(539-654)

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-523)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(3-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZPQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.344

98.801

0.468