Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   NMD29_RS09460 Genome accession   NZ_CP100996
Coordinates   1935189..1936613 (-) Length   474 a.a.
NCBI ID   WP_247153148.1    Uniprot ID   -
Organism   Escherichia coli strain ET151     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1930189..1941613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD29_RS09430 (NMD29_09390) yeeZ 1930244..1931068 (+) 825 WP_000754749.1 SDR family oxidoreductase -
  NMD29_RS09435 (NMD29_09395) yeeY 1931114..1932043 (+) 930 WP_104772125.1 LysR substrate-binding domain-containing protein -
  NMD29_RS09440 (NMD29_09400) yoeI 1932258..1932320 (+) 63 WP_010723108.1 membrane protein YoeI -
  NMD29_RS09445 (NMD29_09405) plaP 1932310..1933668 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  NMD29_RS09450 (NMD29_09410) tsuA 1933847..1934905 (+) 1059 WP_000492315.1 thiosulfate utilization transporter TsuA/YeeE -
  NMD29_RS09455 (NMD29_09415) tsuB 1934919..1935146 (+) 228 WP_000985280.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  NMD29_RS09460 (NMD29_09420) sbcB 1935189..1936613 (-) 1425 WP_247153148.1 exodeoxyribonuclease I Machinery gene
  NMD29_RS09465 (NMD29_09425) - 1936795..1937973 (+) 1179 WP_326390071.1 site-specific integrase -
  NMD29_RS09470 (NMD29_09430) - 1937954..1938145 (-) 192 WP_166430243.1 AlpA family transcriptional regulator -
  NMD29_RS09475 (NMD29_09435) - 1938223..1938567 (-) 345 WP_001281192.1 hypothetical protein -
  NMD29_RS09480 (NMD29_09440) - 1938668..1938847 (-) 180 WP_001277767.1 Eag protein -
  NMD29_RS09485 (NMD29_09445) - 1938944..1939474 (-) 531 WP_241407858.1 dATP/dGTP pyrophosphohydrolase domain-containing protein -
  NMD29_RS09490 (NMD29_09450) - 1939448..1939573 (-) 126 Protein_1855 DUF550 domain-containing protein -
  NMD29_RS09495 (NMD29_09455) - 1939575..1940108 (-) 534 WP_370613190.1 ead/Ea22-like family protein -
  NMD29_RS09500 (NMD29_09460) - 1940158..1940457 (-) 300 WP_096976364.1 hypothetical protein -
  NMD29_RS09505 (NMD29_09465) - 1940454..1940621 (-) 168 WP_001214453.1 DUF2737 family protein -
  NMD29_RS09510 (NMD29_09470) - 1940638..1940952 (-) 315 WP_000753560.1 hypothetical protein -
  NMD29_RS09515 (NMD29_09475) - 1940964..1941446 (-) 483 WP_000041328.1 siphovirus Gp157 family protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54353.66 Da        Isoelectric Point: 5.4740

>NTDB_id=608423 NMD29_RS09460 WP_247153148.1 1935189..1936613(-) (sbcB) [Escherichia coli strain ET151]
MTGTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAIPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=608423 NMD29_RS09460 WP_247153148.1 1935189..1936613(-) (sbcB) [Escherichia coli strain ET151]
ATGACAGGCACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTG
ATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGAATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGTCTGTTTGAATATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCATTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGTTGGCT
GGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(211-471)

Exonuclease (RNase_T)

(12-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.511

99.156

0.58