Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NMD30_RS06300 Genome accession   NZ_CP100994
Coordinates   1289973..1291976 (-) Length   667 a.a.
NCBI ID   WP_000087280.1    Uniprot ID   P33570
Organism   Escherichia coli strain ET201     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1284973..1296976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS06285 (NMD30_06250) aegA 1286105..1288084 (+) 1980 WP_001078871.1 formate-dependent uric acid utilization protein AegA -
  NMD30_RS06290 (NMD30_06255) nudK 1288152..1288727 (+) 576 WP_001299058.1 GDP-mannose pyrophosphatase NudK -
  NMD30_RS06295 (NMD30_06260) ypfG 1288853..1289896 (+) 1044 WP_001270524.1 DUF1176 domain-containing protein -
  NMD30_RS06300 (NMD30_06265) recP/tkt 1289973..1291976 (-) 2004 WP_000087280.1 transketolase Machinery gene
  NMD30_RS06305 (NMD30_06270) tal 1291996..1292946 (-) 951 WP_001003709.1 transaldolase -
  NMD30_RS06310 (NMD30_06275) maeB 1293235..1295514 (+) 2280 WP_000342642.1 NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase -
  NMD30_RS06315 (NMD30_06280) eutS 1295807..1296142 (+) 336 WP_000356956.1 ethanolamine utilization microcompartment protein EutS -
  NMD30_RS06320 (NMD30_06285) eutP 1296155..1296634 (+) 480 WP_000820764.1 ethanolamine utilization acetate kinase EutP -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73042.72 Da        Isoelectric Point: 6.2536

>NTDB_id=608338 NMD30_RS06300 WP_000087280.1 1289973..1291976(-) (recP/tkt) [Escherichia coli strain ET201]
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=608338 NMD30_RS06300 WP_000087280.1 1289973..1291976(-) (recP/tkt) [Escherichia coli strain ET201]
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGTTATACGCCAGG
CGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGG
CGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACTGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATG
AAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGCACAGTAATTGGCTTCGGTTCGCCTAACAAAGCGGGTAAGGAAGAGGCGCATGGCGCACCGCTGGGTGAAGA
AGAAGTGGCATTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAAATCCCTAAAGAGATTTATCACGCCTGGG
ATGCCCGCGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTG
GCAGAAGAGTTTACCCGTCGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
GCAGGCGAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCGATGCTACCGGAGC
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCAGCG
GGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCCTGGGCGAAGATGGTCCGACGCACCAGGCTGTTGAGCAACTGGCCAGC
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCGGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATATTATTCTGATTGCCACCGGTTCAGAGATG
GAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTA
TTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGC
CTGA

Domains

Predicted by InterProScan.

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(3-333)

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-523)

Transketolase, C-terminal domain (Transketolase_C)

(539-654)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P33570

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.496

98.801

0.469