Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD31_RS19830 Genome accession   NZ_CP100987
Coordinates   4014888..4015328 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET210     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4009888..4020328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD31_RS19810 (NMD31_19720) aroP 4010886..4012259 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD31_RS19815 (NMD31_19725) ampE 4012302..4013156 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NMD31_RS19820 (NMD31_19730) ampD 4013153..4013704 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD31_RS19825 (NMD31_19735) nadC 4013792..4014685 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD31_RS19830 (NMD31_19740) pilA 4014888..4015328 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD31_RS19835 (NMD31_19745) pilB 4015338..4016723 (+) 1386 WP_001025147.1 type II secretion system protein GspE Machinery gene
  NMD31_RS19840 (NMD31_19750) hofC 4016713..4017915 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD31_RS19845 (NMD31_19755) guaC 4017950..4018993 (-) 1044 WP_001217338.1 GMP reductase -
  NMD31_RS19850 (NMD31_19760) - 4019149..4019193 (-) 45 WP_120795372.1 protein YacM -
  NMD31_RS19855 (NMD31_19765) coaE 4019218..4019838 (+) 621 WP_001269532.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=608288 NMD31_RS19830 WP_000360904.1 4014888..4015328(+) (pilA) [Escherichia coli strain ET210]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=608288 NMD31_RS19830 WP_000360904.1 4014888..4015328(+) (pilA) [Escherichia coli strain ET210]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363