Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   NMD35_RS21730 Genome accession   NZ_CP100973
Coordinates   4535641..4537470 (-) Length   609 a.a.
NCBI ID   WP_001617666.1    Uniprot ID   -
Organism   Escherichia coli strain ET300     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4530641..4542470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD35_RS21705 (NMD35_21560) bioP 4531359..4532258 (-) 900 WP_001196238.1 biotin transporter -
  NMD35_RS21710 (NMD35_21565) yigL 4532334..4533134 (-) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  NMD35_RS21715 (NMD35_21570) pldB 4533142..4534164 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  NMD35_RS21720 (NMD35_21575) rhtB 4534275..4534895 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  NMD35_RS21725 (NMD35_21580) rhtC 4534957..4535577 (-) 621 WP_000928824.1 threonine export protein RhtC -
  NMD35_RS21730 (NMD35_21585) recQ 4535641..4537470 (-) 1830 WP_001617666.1 ATP-dependent DNA helicase RecQ Machinery gene
  NMD35_RS21735 (NMD35_21590) pldA 4537603..4538472 (-) 870 WP_001259700.1 phospholipase A -
  NMD35_RS21740 (NMD35_21595) yigI 4538637..4539104 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  NMD35_RS21745 (NMD35_21600) rarD 4539156..4540043 (+) 888 WP_000339103.1 EamA family transporter RarD -
  NMD35_RS21750 (NMD35_21605) - 4540090..4540389 (-) 300 WP_000526434.1 hypothetical protein -
  NMD35_RS21755 (NMD35_21610) - 4541015..4541161 (-) 147 Protein_4242 CorA family divalent cation transporter -
  NMD35_RS21760 (NMD35_21615) - 4541348..4542196 (+) 849 WP_001059699.1 YPO3983 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68376.51 Da        Isoelectric Point: 7.2543

>NTDB_id=608059 NMD35_RS21730 WP_001617666.1 4535641..4537470(-) (recQ) [Escherichia coli strain ET300]
MAQADVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=608059 NMD35_RS21730 WP_001617666.1 4535641..4537470(-) (recQ) [Escherichia coli strain ET300]
GTGGCGCAGGCGGATGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAACAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCTT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTACTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGTTTTGGTATGGGCTATGTGGTGGAAGTGATTCGCGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTAAAAGTCTACGGTATGGGCCGTGATAAAAGTCATGAACATTGGGTGAGCGTGATCCGCCAGTTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTCCAACTGACAGAGGCCGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391