Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NMD35_RS06830 Genome accession   NZ_CP100973
Coordinates   1410169..1412172 (-) Length   667 a.a.
NCBI ID   WP_000087298.1    Uniprot ID   A7ZPQ7
Organism   Escherichia coli strain ET300     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1405169..1417172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD35_RS06815 (NMD35_06780) aegA 1406302..1408281 (+) 1980 WP_001078875.1 formate-dependent uric acid utilization protein AegA -
  NMD35_RS06820 (NMD35_06785) nudK 1408349..1408924 (+) 576 WP_000193711.1 GDP-mannose pyrophosphatase NudK -
  NMD35_RS06825 (NMD35_06790) ypfG 1409050..1410093 (+) 1044 WP_001270536.1 DUF1176 domain-containing protein -
  NMD35_RS06830 (NMD35_06795) recP/tkt 1410169..1412172 (-) 2004 WP_000087298.1 transketolase Machinery gene
  NMD35_RS06835 (NMD35_06800) tal 1412192..1413142 (-) 951 WP_001003709.1 transaldolase -
  NMD35_RS06840 (NMD35_06805) maeB 1413431..1415710 (+) 2280 WP_000342642.1 NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase -
  NMD35_RS06845 (NMD35_06810) eutS 1416003..1416338 (+) 336 WP_000356956.1 ethanolamine utilization microcompartment protein EutS -
  NMD35_RS06850 (NMD35_06815) eutP 1416351..1416830 (+) 480 WP_000820764.1 ethanolamine utilization acetate kinase EutP -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73041.78 Da        Isoelectric Point: 6.3824

>NTDB_id=608016 NMD35_RS06830 WP_000087298.1 1410169..1412172(-) (recP/tkt) [Escherichia coli strain ET300]
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=608016 NMD35_RS06830 WP_000087298.1 1410169..1412172(-) (recP/tkt) [Escherichia coli strain ET300]
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAATCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGG
CGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGG
CGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATG
AAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGA
AGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAAAGATTTATCACGCCTGGG
ATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTG
GCAGAAGAGTTTACCCGACGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
ACAGGCAAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCGATGCTGCCTGAGT
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCAGCG
GGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCCTGGGCGAAGATGGTCCGACGCACCAGGCTGTTGAGCAACTGGCCAGC
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCGGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATTCTCTCCAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATATTATTTTGATTGCCACCGGTTCAGAGATG
GAAATCACCCTGCAAGCGGCAGAGAAATTAGCGGGAGAAGGTCGCAATGTTCGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTA
TTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGC
CTGA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(539-654)

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-523)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(3-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZPQ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.344

98.801

0.468