Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NMD35_RS04265 Genome accession   NZ_CP100973
Coordinates   883089..885080 (+) Length   663 a.a.
NCBI ID   WP_000098614.1    Uniprot ID   B7UHY1
Organism   Escherichia coli strain ET300     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 878089..890080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD35_RS04250 (NMD35_04225) - 880060..880791 (+) 732 WP_000758915.1 hypothetical protein -
  NMD35_RS04255 (NMD35_04230) speB 880927..881847 (+) 921 WP_000105559.1 agmatinase -
  NMD35_RS04260 (NMD35_04235) loiP 882053..882811 (-) 759 WP_000701841.1 metalloprotease LoiP -
  NMD35_RS04265 (NMD35_04240) recP/tkt 883089..885080 (+) 1992 WP_000098614.1 transketolase Machinery gene
  NMD35_RS04270 (NMD35_04245) cmtB 885394..885837 (+) 444 WP_001239650.1 PTS mannitol transporter subunit IIA -
  NMD35_RS04275 (NMD35_04250) cmtA 885865..887253 (+) 1389 WP_000428817.1 PTS mannitol transporter subunit IICB -
  NMD35_RS04280 (NMD35_04255) yggP 887268..888545 (+) 1278 WP_000853221.1 zinc-binding dehydrogenase -
  NMD35_RS04285 (NMD35_04260) glpX 888542..889507 (+) 966 WP_000987277.1 class II fructose-bisphosphatase -
  NMD35_RS04290 (NMD35_04265) fumE 889529..890038 (+) 510 WP_000224138.1 fumarase E -

Sequence

Protein


Download         Length: 663 a.a.        Molecular weight: 72211.74 Da        Isoelectric Point: 5.4041

>NTDB_id=608007 NMD35_RS04265 WP_000098614.1 883089..885080(+) (recP/tkt) [Escherichia coli strain ET300]
MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDFLKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGY
DLPMEELKNFRQLHSKTPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDGHDAASIKRAVEEARAVTDKPSL
LMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQLGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQ
EAAEFTRRMKGEMPSDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSGSKAINEDA
AGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAALMKQRQVMVYTHDSIGLGEDGPTHQPVEQVA
SLRVTPNMSTWRPCDQVESAVAWKYGVERQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSE
VELAVAAYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVGLNGAIVGMTTFGESAP
AELLFEEFGFTVDNVVAKAKELL

Nucleotide


Download         Length: 1992 bp        

>NTDB_id=608007 NMD35_RS04265 WP_000098614.1 883089..885080(+) (recP/tkt) [Escherichia coli strain ET300]
ATGTCCTCACGTAAAGAGCTTGCCAATGCTATTCGTGCGCTGAGCATGGACGCAGTACAGAAAGCCAAATCCGGTCACCC
GGGTGCCCCTATGGGTATGGCTGACATTGCCGAAGTCCTGTGGCGTGATTTCCTGAAACACAACCCGCAGAATCCGTCCT
GGGCTGACCGTGACCGCTTCGTGCTGTCCAACGGCCACGGCTCCATGCTGATCTACAGCCTGCTGCACCTCACCGGTTAC
GATCTGCCGATGGAAGAACTGAAAAACTTCCGTCAGCTGCACTCTAAAACTCCGGGTCACCCGGAAGTGGGTTACACCGC
TGGTGTGGAAACCACCACCGGTCCGCTGGGTCAGGGTATTGCCAACGCAGTCGGTATGGCGATTGCAGAAAAAACGCTGG
CGGCGCAGTTTAACCGTCCGGGCCACGACATTGTCGACCACTACACCTATGCCTTCATGGGCGACGGCTGCATGATGGAA
GGCATCTCCCACGAAGTTTGCTCTCTGGCGGGTACGCTGAAGCTGGGTAAACTGATTGCGTTCTACGATGACAACGGTAT
CTCTATTGATGGTCACGTTGAAGGCTGGTTCACCGACGACACCGCAATGCGTTTCGAAGCTTACGGCTGGCACGTTATTC
GCGACATCGACGGTCATGACGCGGCATCCATCAAACGCGCAGTAGAAGAAGCGCGCGCAGTGACTGACAAACCGTCCCTG
CTGATGTGCAAAACCATCATCGGTTTCGGTTCCCCGAACAAAGCCGGTACTCACGACTCCCACGGTGCGCCGCTGGGCGA
CGCTGAAATTGCCCTGACCCGCGAACAGCTGGGCTGGAAATATGCACCGTTCGAAATCCCGTCTGAAATCTATGCTCAGT
GGGATGCGAAAGAAGCAGGCCAGGCGAAAGAATCCGCATGGAACGAGAAATTCGCTGCTTACGCGAAAGCTTATCCGCAG
GAAGCCGCTGAATTTACCCGCCGTATGAAAGGCGAAATGCCGTCTGACTTCGACGCGAAAGCAAAAGAGTTCATTGCTAA
ACTGCAGGCTAACCCGGCGAAAATCGCCAGCCGTAAAGCGTCTCAGAATGCTATCGAAGCTTTCGGTCCGCTGTTGCCGG
AATTCCTCGGCGGTTCTGCTGACCTGGCACCGTCTAACCTGACCCTGTGGTCTGGTTCTAAAGCAATCAACGAAGATGCT
GCGGGTAACTACATCCACTACGGTGTTCGCGAGTTCGGTATGACCGCGATTGCTAACGGTATCTCCCTGCACGGTGGTTT
CCTGCCGTACACCTCCACCTTCCTGATGTTCGTGGAATACGCACGTAACGCCGTACGTATGGCTGCGCTGATGAAACAGC
GTCAGGTGATGGTTTACACCCACGACTCCATCGGTCTGGGCGAAGATGGCCCGACTCACCAGCCGGTAGAGCAGGTCGCT
TCTCTGCGCGTAACCCCGAACATGTCTACATGGCGTCCGTGTGACCAGGTTGAATCCGCGGTCGCGTGGAAATACGGTGT
TGAGCGTCAGGACGGCCCGACCGCACTGATCCTCTCCCGTCAGAACCTGGCGCAGCAGGAACGAACTGAAGAGCAACTGG
CAAACATCGCGCGCGGTGGTTATGTGCTGAAAGACTGCGCCGGTCAGCCGGAACTGATTTTCATCGCTACCGGTTCAGAA
GTTGAACTGGCTGTTGCTGCCTACGAAAAACTGACTGCCGAAGGCGTGAAAGCGCGCGTGGTGTCCATGCCGTCTACCGA
CGCATTTGACAAGCAGGATGCTGCTTACCGTGAATCCGTACTGCCGAAAGCGGTTACTGCACGCGTTGCTGTAGAAGCGG
GTATTGCTGACTACTGGTACAAGTATGTTGGCCTGAACGGTGCTATCGTCGGTATGACCACTTTCGGTGAATCTGCTCCG
GCAGAGCTGCTGTTTGAAGAGTTCGGCTTCACTGTTGATAACGTTGTTGCGAAAGCAAAAGAACTGCTGTAA

Domains

Predicted by InterProScan.

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UHY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

48.024

99.246

0.477