Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   LAZ98_RS09960 Genome accession   NZ_CP083764
Coordinates   1955527..1955961 (+) Length   144 a.a.
NCBI ID   WP_224443691.1    Uniprot ID   -
Organism   Bacillus velezensis strain DMB07     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1950527..1960961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ98_RS09955 (LAZ98_09955) - 1954027..1955238 (-) 1212 WP_026092252.1 cytochrome P450 -
  LAZ98_RS09960 (LAZ98_09960) nucA/comI 1955527..1955961 (+) 435 WP_224443691.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  LAZ98_RS09965 (LAZ98_09965) - 1956021..1956776 (+) 756 WP_022553315.1 YoaK family protein -
  LAZ98_RS09970 (LAZ98_09970) - 1956810..1957172 (-) 363 WP_007410383.1 hypothetical protein -
  LAZ98_RS09975 (LAZ98_09975) - 1957364..1958692 (-) 1329 WP_017417817.1 S8 family peptidase -
  LAZ98_RS09980 (LAZ98_09980) - 1958871..1959104 (+) 234 WP_015239897.1 hypothetical protein -
  LAZ98_RS09985 (LAZ98_09985) - 1959360..1960067 (+) 708 WP_224443692.1 poly-gamma-glutamate hydrolase family protein -
  LAZ98_RS09990 (LAZ98_09990) - 1960127..1960579 (+) 453 WP_014305039.1 OsmC family protein -
  LAZ98_RS09995 (LAZ98_09995) - 1960617..1960946 (-) 330 WP_032865393.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15604.57 Da        Isoelectric Point: 7.2409

>NTDB_id=607825 LAZ98_RS09960 WP_224443691.1 1955527..1955961(+) (nucA/comI) [Bacillus velezensis strain DMB07]
MNAFMKWAASLLLVIYLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=607825 LAZ98_RS09960 WP_224443691.1 1955527..1955961(+) (nucA/comI) [Bacillus velezensis strain DMB07]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTATCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486