Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K8M08_RS21000 Genome accession   NZ_CP083749
Coordinates   4035839..4036273 (+) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus sp. CRB-7     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4030839..4041273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8M08_RS20980 (K8M08_20980) - 4031733..4032890 (-) 1158 WP_000434566.1 aminotransferase A -
  K8M08_RS20985 (K8M08_20985) - 4033015..4033215 (-) 201 WP_000929285.1 hypothetical protein -
  K8M08_RS20990 (K8M08_20990) kinB 4033525..4034799 (+) 1275 WP_000420393.1 sporulation sensor histidine kinase KinB -
  K8M08_RS20995 (K8M08_20995) - 4034853..4035602 (-) 750 WP_000388474.1 DUF3967 domain-containing protein -
  K8M08_RS21000 (K8M08_21000) nucA/comI 4035839..4036273 (+) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  K8M08_RS21005 (K8M08_21005) comJ 4036310..4036699 (+) 390 WP_000424012.1 competence protein ComJ -
  K8M08_RS21010 (K8M08_21010) metE 4036737..4039025 (-) 2289 WP_001007599.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  K8M08_RS21015 (K8M08_21015) - 4039694..4040251 (-) 558 WP_000062074.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=607670 K8M08_RS21000 WP_000811502.1 4035839..4036273(+) (nucA/comI) [Bacillus sp. CRB-7]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=607670 K8M08_RS21000 WP_000811502.1 4035839..4036273(+) (nucA/comI) [Bacillus sp. CRB-7]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCATATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTGTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCATTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACACATTGAATATATAAGTCCAGCAGATAACCGTGGAGCAGGGTCTTGGGTAGGAAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549