Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K8M08_RS13145 Genome accession   NZ_CP083749
Coordinates   2562564..2563940 (-) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus sp. CRB-7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2557564..2568940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8M08_RS13125 (K8M08_13125) ispF 2558927..2559403 (-) 477 WP_000488394.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  K8M08_RS13130 (K8M08_13130) ispD 2559519..2560199 (-) 681 WP_000288291.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  K8M08_RS13135 (K8M08_13135) - 2560216..2561325 (-) 1110 WP_000919683.1 PIN/TRAM domain-containing protein -
  K8M08_RS13140 (K8M08_13140) disA 2561487..2562560 (-) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  K8M08_RS13145 (K8M08_13145) radA 2562564..2563940 (-) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  K8M08_RS13150 (K8M08_13150) clpC 2564037..2566472 (-) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  K8M08_RS13155 (K8M08_13155) - 2566495..2567559 (-) 1065 WP_000050825.1 protein arginine kinase -
  K8M08_RS13160 (K8M08_13160) - 2567564..2568112 (-) 549 WP_000128380.1 UvrB/UvrC motif-containing protein -
  K8M08_RS13165 (K8M08_13165) ctsR 2568286..2568747 (-) 462 WP_001244563.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=607645 K8M08_RS13145 WP_001085212.1 2562564..2563940(-) (radA) [Bacillus sp. CRB-7]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=607645 K8M08_RS13145 WP_001085212.1 2562564..2563940(-) (radA) [Bacillus sp. CRB-7]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTTATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATTTACCTGAAGTAACGTCAGC
GCCAGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTCGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCGATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCGGAAGTATTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTGGCGG
GGTCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCGACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGGTTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACCATTCCAGATGGGATCGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629