Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD42_RS18890 Genome accession   NZ_CP100950
Coordinates   3865349..3865789 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain ET479     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3860349..3870789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD42_RS18870 (NMD42_18725) aroP 3861347..3862720 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  NMD42_RS18875 (NMD42_18730) ampE 3862763..3863617 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD42_RS18880 (NMD42_18735) ampD 3863614..3864165 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD42_RS18885 (NMD42_18740) nadC 3864253..3865146 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD42_RS18890 (NMD42_18745) pilA 3865349..3865789 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NMD42_RS18895 (NMD42_18750) pilB 3865799..3867184 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  NMD42_RS18900 (NMD42_18755) hofC 3867174..3868376 (+) 1203 WP_000157234.1 protein transport protein HofC -
  NMD42_RS18905 (NMD42_18760) guaC 3868411..3869454 (-) 1044 WP_001217338.1 GMP reductase -
  NMD42_RS18910 (NMD42_18765) - 3869610..3869654 (-) 45 WP_120795372.1 protein YacM -
  NMD42_RS18915 (NMD42_18770) coaE 3869679..3870299 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=607637 NMD42_RS18890 WP_000360895.1 3865349..3865789(+) (pilA) [Escherichia coli strain ET479]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=607637 NMD42_RS18890 WP_000360895.1 3865349..3865789(+) (pilA) [Escherichia coli strain ET479]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363