Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LBF07_RS09000 Genome accession   NZ_CP083709
Coordinates   1832360..1833793 (+) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter cloacae complex sp. ECL352     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827360..1838793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LBF07_RS08970 (LBF07_08970) - 1827927..1828904 (+) 978 WP_094934467.1 calcium/sodium antiporter -
  LBF07_RS08975 (LBF07_08975) kdsD 1828918..1829904 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  LBF07_RS08980 (LBF07_08980) kdsC 1829919..1830485 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LBF07_RS08985 (LBF07_08985) lptC 1830482..1831057 (+) 576 WP_033146749.1 LPS export ABC transporter periplasmic protein LptC -
  LBF07_RS08990 (LBF07_08990) lptA 1831026..1831580 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LBF07_RS08995 (LBF07_08995) lptB 1831587..1832312 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  LBF07_RS09000 (LBF07_09000) rpoN/rpoN1 1832360..1833793 (+) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  LBF07_RS09005 (LBF07_09005) hpf 1833816..1834103 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  LBF07_RS09010 (LBF07_09010) ptsN 1834186..1834677 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LBF07_RS09015 (LBF07_09015) rapZ 1834723..1835577 (+) 855 WP_014885397.1 RNase adapter RapZ -
  LBF07_RS09020 (LBF07_09020) npr 1835574..1835846 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LBF07_RS09025 (LBF07_09025) mtgA 1835886..1836611 (-) 726 WP_094934468.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LBF07_RS09030 (LBF07_09030) elbB 1836608..1837261 (-) 654 WP_094934469.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=607361 LBF07_RS09000 WP_023309360.1 1832360..1833793(+) (rpoN/rpoN1) [Enterobacter cloacae complex sp. ECL352]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=607361 LBF07_RS09000 WP_023309360.1 1832360..1833793(+) (rpoN/rpoN1) [Enterobacter cloacae complex sp. ECL352]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTATCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATTATTAGCGATCATCTGGATCTGCTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTAAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAAACCAG
CGAGCCCGAGTACGTGATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48