Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD46_RS01325 Genome accession   NZ_CP100936
Coordinates   280712..282208 (+) Length   498 a.a.
NCBI ID   WP_001040683.1    Uniprot ID   -
Organism   Escherichia coli strain ET650     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275712..287208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD46_RS01295 (NMD46_01280) tusA 275959..276204 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NMD46_RS01300 (NMD46_01285) zntA 276306..278504 (-) 2199 WP_044066813.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NMD46_RS01305 (NMD46_01290) yhhN 278578..279204 (-) 627 WP_154761731.1 lysoplasmalogenase -
  NMD46_RS01310 (NMD46_01295) yhhM 279345..279704 (+) 360 WP_000042903.1 DUF2500 domain-containing protein -
  NMD46_RS01315 (NMD46_01300) yhhL 279707..279976 (-) 270 WP_001295207.1 DUF1145 family protein -
  NMD46_RS01320 (NMD46_01305) rsmD 279966..280562 (-) 597 WP_370603795.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NMD46_RS01325 (NMD46_01310) pilA 280712..282208 (+) 1497 WP_001040683.1 signal recognition particle-docking protein FtsY Machinery gene
  NMD46_RS01330 (NMD46_01315) ftsE 282211..282879 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NMD46_RS01335 (NMD46_01320) ftsX 282872..283930 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NMD46_RS01340 (NMD46_01325) rpoH 284175..285029 (+) 855 WP_370603797.1 RNA polymerase sigma factor RpoH -
  NMD46_RS01345 (NMD46_01330) livJ 285300..286403 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NMD46_RS01350 - 286463..286562 (+) 100 Protein_267 hypothetical protein -
  NMD46_RS01355 (NMD46_01335) panM 286602..286985 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54617.41 Da        Isoelectric Point: 4.1560

>NTDB_id=607258 NMD46_RS01325 WP_001040683.1 280712..282208(+) (pilA) [Escherichia coli strain ET650]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAGVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=607258 NMD46_RS01325 WP_001040683.1 280712..282208(+) (pilA) [Escherichia coli strain ET650]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAATCGGTTGTAGAAGAAATCGTTCAGGTGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGGAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCAGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378