Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD47_RS19430 Genome accession   NZ_CP100933
Coordinates   3958510..3958950 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3953510..3963950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD47_RS19410 (NMD47_19280) aroP 3954508..3955881 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD47_RS19415 (NMD47_19285) ampE 3955924..3956778 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NMD47_RS19420 (NMD47_19290) ampD 3956775..3957326 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD47_RS19425 (NMD47_19295) nadC 3957414..3958307 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD47_RS19430 (NMD47_19300) pilA 3958510..3958950 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD47_RS19435 (NMD47_19305) pilB 3958960..3960345 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD47_RS19440 (NMD47_19310) hofC 3960335..3961537 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD47_RS19445 (NMD47_19315) guaC 3961572..3962615 (-) 1044 WP_001217337.1 GMP reductase -
  NMD47_RS19450 (NMD47_19320) - 3962771..3962815 (-) 45 WP_120795372.1 protein YacM -
  NMD47_RS19455 (NMD47_19325) coaE 3962840..3963460 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=607229 NMD47_RS19430 WP_000360904.1 3958510..3958950(+) (pilA) [Escherichia coli strain ET665]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=607229 NMD47_RS19430 WP_000360904.1 3958510..3958950(+) (pilA) [Escherichia coli strain ET665]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363