Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LA331_RS13225 Genome accession   NZ_CP083658
Coordinates   2712858..2713658 (+) Length   266 a.a.
NCBI ID   WP_005232134.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain FDAARGOS_1487     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2707858..2718658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA331_RS13205 (LA331_13205) - 2710105..2710656 (+) 552 WP_004782094.1 phosphatase PAP2 family protein -
  LA331_RS13210 (LA331_13210) iscX 2710745..2710945 (+) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  LA331_RS13215 (LA331_13215) ndk 2711055..2711486 (+) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  LA331_RS13220 (LA331_13220) rlmN 2711608..2712843 (+) 1236 WP_005232136.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  LA331_RS13225 (LA331_13225) pilF 2712858..2713658 (+) 801 WP_005232134.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  LA331_RS13230 (LA331_13230) - 2713649..2714434 (+) 786 WP_005232131.1 helix-turn-helix domain-containing protein -
  LA331_RS13235 (LA331_13235) ispG 2714458..2715579 (+) 1122 WP_005232130.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  LA331_RS13240 (LA331_13240) hisS 2715583..2716878 (+) 1296 WP_005232127.1 histidine--tRNA ligase -
  LA331_RS13245 (LA331_13245) - 2716909..2717607 (+) 699 WP_005232125.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29990.11 Da        Isoelectric Point: 9.0592

>NTDB_id=607202 LA331_RS13225 WP_005232134.1 2712858..2713658(+) (pilF) [Acinetobacter variabilis strain FDAARGOS_1487]
MSNLTSKLALVSVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVL
LQQEGSKPNLEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDL
VNAEKSFKQALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGVGQKNQSARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=607202 LA331_RS13225 WP_005232134.1 2712858..2713658(+) (pilF) [Acinetobacter variabilis strain FDAARGOS_1487]
TTGAGTAATCTGACATCTAAACTTGCTTTGGTTTCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGA
TCTGGGTCAGAAGGACCCGGAAAAAGCCACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGG
ATTCAGCTAAACGTGCGCTGGATCAGGCACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTG
CTTCAGCAGGAAGGCAGCAAACCTAATCTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCCAAAAA
TGCCCAGGCACGAAATAACTACGGCACCTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTG
CCGGCGCAACTTTAGGTTATGACCAGCGATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATTTG
GTGAATGCTGAAAAATCATTCAAACAGGCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAAT
TTTTTATCTGAACCAGCAGTTCCCGGCCGCGACTCAAATGTATGAACAGTTTGTACGTGGCGTGGGGCAGAAAAACCAGA
GTGCCCGTGCGCTCTGGATTGGCATTCGCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTATTGGTCAATCAA
TTACGTGCATTGTTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(145-174)

Tetratricopeptide repeat (TPR_14)

(40-79)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.692

100

0.767
  pilF Acinetobacter baylyi ADP1

69.663

100

0.699