Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LA325_RS20080 Genome accession   NZ_CP083655
Coordinates   2198019..2199524 (+) Length   501 a.a.
NCBI ID   WP_045578772.1    Uniprot ID   -
Organism   Burkholderia vietnamiensis strain FDAARGOS_1488     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2193019..2204524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA325_RS20055 (LA325_20050) kdsD 2194260..2195243 (+) 984 WP_014723665.1 arabinose 5-phosphate isomerase KdsD -
  LA325_RS20060 (LA325_20055) - 2195243..2195779 (+) 537 WP_011886052.1 KdsC family phosphatase -
  LA325_RS20065 (LA325_20060) lptC 2195783..2196397 (+) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  LA325_RS20070 (LA325_20065) lptA 2196418..2197083 (+) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  LA325_RS20075 (LA325_20070) lptB 2197080..2197856 (+) 777 WP_011886055.1 LPS export ABC transporter ATP-binding protein -
  LA325_RS20080 (LA325_20075) rpoN/rpoN1 2198019..2199524 (+) 1506 WP_045578772.1 RNA polymerase factor sigma-54 Machinery gene
  LA325_RS20085 (LA325_20080) hpf 2199683..2200042 (+) 360 WP_011886057.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LA325_RS20090 (LA325_20085) - 2200237..2200749 (+) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  LA325_RS20095 (LA325_20090) hprK 2200879..2201847 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  LA325_RS20100 (LA325_20095) rapZ 2201923..2202831 (+) 909 WP_034192566.1 RNase adapter RapZ -
  LA325_RS20105 (LA325_20100) - 2202859..2203497 (+) 639 WP_011886060.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54828.98 Da        Isoelectric Point: 4.8247

>NTDB_id=607157 LA325_RS20080 WP_045578772.1 2198019..2199524(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain FDAARGOS_1488]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDTSDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=607157 LA325_RS20080 WP_045578772.1 2198019..2199524(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain FDAARGOS_1488]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCGAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACACGAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTCCAGGAAGCCGCCACATCGCTGCGCGAGCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTGGAAGTCGACTGCGACGAGCTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTGGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGCCGCGGCGATCCGTCGACCGGATCGTTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.836

100

0.695