Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD48_RS19235 Genome accession   NZ_CP100930
Coordinates   3863791..3864231 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET670     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3858791..3869231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD48_RS19215 (NMD48_19090) aroP 3859789..3861162 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD48_RS19220 (NMD48_19095) ampE 3861205..3862059 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD48_RS19225 (NMD48_19100) ampD 3862056..3862607 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD48_RS19230 (NMD48_19105) nadC 3862695..3863588 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD48_RS19235 (NMD48_19110) pilA 3863791..3864231 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD48_RS19240 (NMD48_19115) pilB 3864241..3865626 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD48_RS19245 (NMD48_19120) hofC 3865616..3866818 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD48_RS19250 (NMD48_19125) guaC 3866853..3867896 (-) 1044 WP_001217338.1 GMP reductase -
  NMD48_RS19255 (NMD48_19130) - 3868052..3868096 (-) 45 WP_120795372.1 protein YacM -
  NMD48_RS19260 (NMD48_19135) coaE 3868121..3868741 (+) 621 WP_016232452.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=607147 NMD48_RS19235 WP_000360904.1 3863791..3864231(+) (pilA) [Escherichia coli strain ET670]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=607147 NMD48_RS19235 WP_000360904.1 3863791..3864231(+) (pilA) [Escherichia coli strain ET670]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363