Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NMD50_RS02660 Genome accession   NZ_CP100922
Coordinates   543310..544743 (-) Length   477 a.a.
NCBI ID   WP_337235857.1    Uniprot ID   -
Organism   Escherichia coli strain ET734     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538310..549743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD50_RS02625 (NMD50_02625) elbB 539001..539654 (+) 654 WP_001300411.1 isoprenoid biosynthesis glyoxalase ElbB -
  NMD50_RS02630 (NMD50_02630) mtgA 539651..540379 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  NMD50_RS02635 (NMD50_02635) yrbL 540376..541008 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  NMD50_RS02640 (NMD50_02640) npr 541222..541494 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  NMD50_RS02645 (NMD50_02645) rapZ 541491..542345 (-) 855 WP_000243741.1 RNase adapter RapZ -
  NMD50_RS02650 (NMD50_02650) ptsN 542391..542882 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  NMD50_RS02655 (NMD50_02655) hpf 543000..543287 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  NMD50_RS02660 (NMD50_02660) rpoN/rpoN1 543310..544743 (-) 1434 WP_337235857.1 RNA polymerase factor sigma-54 Machinery gene
  NMD50_RS02665 (NMD50_02665) lptB 544791..545516 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  NMD50_RS02670 (NMD50_02670) lptA 545523..546080 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  NMD50_RS02675 (NMD50_02675) lptC 546049..546624 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  NMD50_RS02680 (NMD50_02680) kdsC 546621..547187 (-) 567 WP_332149941.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  NMD50_RS02685 (NMD50_02685) kdsD 547208..548194 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  NMD50_RS02690 (NMD50_02690) yrbG 548208..549185 (-) 978 WP_000922901.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54005.78 Da        Isoelectric Point: 4.3663

>NTDB_id=606938 NMD50_RS02660 WP_337235857.1 543310..544743(-) (rpoN/rpoN1) [Escherichia coli strain ET734]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTSGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
ILESIGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=606938 NMD50_RS02660 WP_337235857.1 543310..544743(-) (rpoN/rpoN1) [Escherichia coli strain ET734]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCTCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATAGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGTCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAACTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463