Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD50_RS01400 Genome accession   NZ_CP100922
Coordinates   296802..298178 (+) Length   458 a.a.
NCBI ID   WP_155555796.1    Uniprot ID   -
Organism   Escherichia coli strain ET734     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291802..303178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD50_RS01370 (NMD50_01360) tusA 292049..292294 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NMD50_RS01375 (NMD50_01365) zntA 292396..294594 (-) 2199 WP_337235553.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NMD50_RS01380 (NMD50_01370) yhhN 294668..295294 (-) 627 WP_000964718.1 lysoplasmalogenase -
  NMD50_RS01385 (NMD50_01375) yhhM 295435..295794 (+) 360 WP_155555797.1 DUF2500 domain-containing protein -
  NMD50_RS01390 (NMD50_01380) yhhL 295797..296066 (-) 270 WP_001311191.1 DUF1145 family protein -
  NMD50_RS01395 (NMD50_01385) rsmD 296056..296652 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NMD50_RS01400 (NMD50_01390) pilA 296802..298178 (+) 1377 WP_155555796.1 signal recognition particle-docking protein FtsY Machinery gene
  NMD50_RS01405 (NMD50_01395) ftsE 298181..298849 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NMD50_RS01410 (NMD50_01400) ftsX 298842..299900 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NMD50_RS01415 (NMD50_01405) rpoH 300145..300999 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NMD50_RS01420 (NMD50_01410) livJ 301270..302373 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NMD50_RS01425 - 302433..302532 (+) 100 Protein_282 hypothetical protein -
  NMD50_RS01430 (NMD50_01415) panM 302573..302956 (-) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50370.99 Da        Isoelectric Point: 4.3056

>NTDB_id=606931 NMD50_RS01400 WP_155555796.1 296802..298178(+) (pilA) [Escherichia coli strain ET734]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARL
KRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEIL
AKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGAD
SASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVG
LTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=606931 NMD50_RS01400 WP_155555796.1 296802..298178(+) (pilA) [Escherichia coli strain ET734]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAACCTGTGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGGCGGTGATGGTGGTTCCTC
CGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTG
AAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGA
TCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGATGTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGA
CGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGCGCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTG
GCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGCCGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAA
AACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCC
GTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAACAATATTCCGGTGATTGCCCAGCATACTGGTGCGGAT
TCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAGCGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACG
CCTGCAGAACAAATCGCATCTGATGGAAGAGTTGAAGAAAATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGC
ATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCTGTTGGC
TTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTAT
CCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTT
TTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(254-454)

SRP54-type protein, helical bundle domain (SRP54_N)

(171-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.317

89.52

0.424