Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LA332_RS21935 Genome accession   NZ_CP083630
Coordinates   4607349..4608782 (-) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain FDAARGOS_1505     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4602349..4613782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA332_RS21905 (LA332_21905) elbB 4603924..4604577 (+) 654 WP_032614491.1 isoprenoid biosynthesis glyoxalase ElbB -
  LA332_RS21910 (LA332_21910) mtgA 4604580..4605299 (+) 720 WP_032614632.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LA332_RS21915 (LA332_21915) npr 4605296..4605568 (-) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  LA332_RS21920 (LA332_21920) rapZ 4605565..4606419 (-) 855 WP_032614487.1 RNase adapter RapZ -
  LA332_RS21925 (LA332_21925) ptsN 4606465..4606956 (-) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LA332_RS21930 (LA332_21930) hpf 4607039..4607326 (-) 288 WP_032614483.1 ribosome hibernation promoting factor -
  LA332_RS21935 (LA332_21935) rpoN/rpoN1 4607349..4608782 (-) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  LA332_RS21940 (LA332_21940) lptB 4608830..4609555 (-) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  LA332_RS21945 (LA332_21945) lptA 4609562..4610137 (-) 576 WP_032614476.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LA332_RS21950 (LA332_21950) lptC 4610106..4610681 (-) 576 WP_032614475.1 LPS export ABC transporter periplasmic protein LptC -
  LA332_RS21955 (LA332_21955) kdsC 4610678..4611244 (-) 567 WP_032614474.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LA332_RS21960 (LA332_21960) kdsD 4611259..4612245 (-) 987 WP_032614472.1 arabinose-5-phosphate isomerase KdsD -
  LA332_RS21965 (LA332_21965) - 4612259..4613236 (-) 978 WP_032614470.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=606909 LA332_RS21935 WP_032614481.1 4607349..4608782(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain FDAARGOS_1505]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=606909 LA332_RS21935 WP_032614481.1 4607349..4608782(-) (rpoN/rpoN1) [Leclercia adecarboxylata strain FDAARGOS_1505]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGCACGCCATCCGGTACGCGCGCTGATTACCAGGATGA
TGAGTTGCCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACACCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCTCGTCTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGGGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACCAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGT
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGTTGCTGCGCGTCAGCCGCTGCAT
CGTGGAGCAGCAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCGTCTACGGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48