Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD53_RS18980 Genome accession   NZ_CP100909
Coordinates   3857233..3857673 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET776     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3852233..3862673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD53_RS18960 (NMD53_18840) aroP 3853231..3854604 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD53_RS18965 (NMD53_18845) ampE 3854647..3855501 (-) 855 WP_021559212.1 beta-lactamase regulator AmpE -
  NMD53_RS18970 (NMD53_18850) ampD 3855498..3856049 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD53_RS18975 (NMD53_18855) nadC 3856137..3857030 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD53_RS18980 (NMD53_18860) pilA 3857233..3857673 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD53_RS18985 (NMD53_18865) pilB 3857683..3859068 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD53_RS18990 (NMD53_18870) hofC 3859058..3860260 (+) 1203 WP_001619105.1 protein transport protein HofC -
  NMD53_RS18995 (NMD53_18875) guaC 3860295..3861338 (-) 1044 WP_001217337.1 GMP reductase -
  NMD53_RS19000 (NMD53_18880) - 3861494..3861538 (-) 45 WP_120795372.1 protein YacM -
  NMD53_RS19005 (NMD53_18885) coaE 3861563..3862183 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=606740 NMD53_RS18980 WP_000360904.1 3857233..3857673(+) (pilA) [Escherichia coli strain ET776]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606740 NMD53_RS18980 WP_000360904.1 3857233..3857673(+) (pilA) [Escherichia coli strain ET776]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363