Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LA316_RS02590 Genome accession   NZ_CP083620
Coordinates   542137..543570 (-) Length   477 a.a.
NCBI ID   WP_028014571.1    Uniprot ID   A0AAU7N964
Organism   Enterobacter bugandensis strain FDAARGOS_1520     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 537137..548570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA316_RS02560 (LA316_02560) elbB 538667..539320 (+) 654 WP_088204346.1 isoprenoid biosynthesis glyoxalase ElbB -
  LA316_RS02565 (LA316_02565) mtgA 539317..540042 (+) 726 WP_049136842.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LA316_RS02570 (LA316_02570) npr 540084..540356 (-) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  LA316_RS02575 (LA316_02575) rapZ 540353..541207 (-) 855 WP_014885397.1 RNase adapter RapZ -
  LA316_RS02580 (LA316_02580) ptsN 541253..541744 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LA316_RS02585 (LA316_02585) hpf 541827..542114 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  LA316_RS02590 (LA316_02590) rpoN/rpoN1 542137..543570 (-) 1434 WP_028014571.1 RNA polymerase factor sigma-54 Machinery gene
  LA316_RS02595 (LA316_02595) lptB 543618..544343 (-) 726 WP_028014570.1 LPS export ABC transporter ATP-binding protein -
  LA316_RS02600 (LA316_02600) lptA 544350..544904 (-) 555 WP_028014569.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LA316_RS02605 (LA316_02605) lptC 544873..545448 (-) 576 WP_028014568.1 LPS export ABC transporter periplasmic protein LptC -
  LA316_RS02610 (LA316_02610) kdsC 545445..546011 (-) 567 WP_025756695.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LA316_RS02615 (LA316_02615) kdsD 546026..547012 (-) 987 WP_028014567.1 arabinose-5-phosphate isomerase KdsD -
  LA316_RS02620 (LA316_02620) - 547026..548003 (-) 978 WP_028014566.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54181.95 Da        Isoelectric Point: 4.3173

>NTDB_id=606691 LA316_RS02590 WP_028014571.1 542137..543570(-) (rpoN/rpoN1) [Enterobacter bugandensis strain FDAARGOS_1520]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIEIEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=606691 LA316_RS02590 WP_028014571.1 542137..543570(-) (rpoN/rpoN1) [Enterobacter bugandensis strain FDAARGOS_1520]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCGATGACGCCGCAGTTACAGCAGGCAATTCGTCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCCCTGCTGGAGCAAGCCGATCTTCATG
ACGAGGTAGATACCCAGCAGTCACAGGACACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTATACCGCCGGGACCCCTTCCGGCACGCGTGCAGACTACCAGGACGA
CGAGCTACCGGTCTACCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTAGAGCTTACCCCCTTCT
CCGATACCGACCGCGCTATTGCCACGTCGATTGTCGACGCCGTTGATGACACCGGTTATTTGACCGTCACGCTGGACGAC
ATTCTGGAAAGCATGGGCGATGAAGAGATTGAGATTGAGGAGATTGAAGCGGTACTGAAACGCATTCAGCGCTTCGATCC
GGTCGGGGTGGCCGCGAAGGACCTGCGCGACTGTCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTCG
ACGAGGCACGCTTAATCATCAGCGATCATCTGGATCTTCTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTAAATCTGATTCAGTCCCTCGATCCGCGTCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTTATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCTGATAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTACGCCTCCATGTGTACCAGCGCGCGCAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGTTGATCAAGAGCCTTGAGAGCCGGAATGATACCCTGCTTCGCGTGAGCCGCTGCAT
CGTCGAACAGCAGCAGGCCTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCCACTATTTCCCGCGTTACTACGCAGAAGTATCTGCACAGTCCGCGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAGGGTGGCGGCGAAGCCTCCTCAACGGCGATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAATCCCGCGAAGCCACTAAGTGACAGTAAGTTGACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48