Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD53_RS01350 Genome accession   NZ_CP100909
Coordinates   293178..294674 (+) Length   498 a.a.
NCBI ID   WP_001040683.1    Uniprot ID   -
Organism   Escherichia coli strain ET776     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288178..299674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD53_RS01320 (NMD53_01310) tusA 288425..288670 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NMD53_RS01325 (NMD53_01315) zntA 288772..290970 (-) 2199 WP_000106560.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NMD53_RS01330 (NMD53_01320) yhhN 291044..291670 (-) 627 WP_000964724.1 lysoplasmalogenase -
  NMD53_RS01335 (NMD53_01325) yhhM 291811..292170 (+) 360 WP_000042900.1 DUF2500 domain-containing protein -
  NMD53_RS01340 (NMD53_01330) yhhL 292173..292442 (-) 270 WP_001295207.1 DUF1145 family protein -
  NMD53_RS01345 (NMD53_01335) rsmD 292432..293028 (-) 597 WP_000743181.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NMD53_RS01350 (NMD53_01340) pilA 293178..294674 (+) 1497 WP_001040683.1 signal recognition particle-docking protein FtsY Machinery gene
  NMD53_RS01355 (NMD53_01345) ftsE 294677..295345 (+) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  NMD53_RS01360 (NMD53_01350) ftsX 295338..296396 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NMD53_RS01365 (NMD53_01355) rpoH 296641..297495 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NMD53_RS01370 (NMD53_01360) livJ 297767..298870 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NMD53_RS01375 - 298930..299029 (+) 100 Protein_272 hypothetical protein -
  NMD53_RS01380 (NMD53_01365) panM 299070..299453 (-) 384 WP_000778792.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54617.41 Da        Isoelectric Point: 4.1560

>NTDB_id=606686 NMD53_RS01350 WP_001040683.1 293178..294674(+) (pilA) [Escherichia coli strain ET776]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAGVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=606686 NMD53_RS01350 WP_001040683.1 293178..294674(+) (pilA) [Escherichia coli strain ET776]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAATCGGTTGTAGAAGAAATCGTTCAGGTGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGGAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378