Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LA353_RS21300 Genome accession   NZ_CP083613
Coordinates   4422251..4423684 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4417251..4428684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA353_RS21270 (LA353_21265) - 4417818..4418795 (+) 978 WP_047051237.1 calcium/sodium antiporter -
  LA353_RS21275 (LA353_21270) kdsD 4418809..4419795 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  LA353_RS21280 (LA353_21275) kdsC 4419810..4420376 (+) 567 WP_047051238.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LA353_RS21285 (LA353_21280) lptC 4420373..4420948 (+) 576 WP_047051239.1 LPS export ABC transporter periplasmic protein LptC -
  LA353_RS21290 (LA353_21285) lptA 4420917..4421471 (+) 555 WP_015571984.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  LA353_RS21295 (LA353_21290) lptB 4421478..4422203 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  LA353_RS21300 (LA353_21295) rpoN/rpoN1 4422251..4423684 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  LA353_RS21305 (LA353_21300) hpf 4423707..4423994 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  LA353_RS21310 (LA353_21305) ptsN 4424077..4424568 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LA353_RS21315 (LA353_21310) rapZ 4424614..4425468 (+) 855 WP_003861870.1 RNase adapter RapZ -
  LA353_RS21320 (LA353_21315) npr 4425465..4425737 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  LA353_RS21325 (LA353_21320) mtgA 4425777..4426502 (-) 726 WP_047051240.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LA353_RS21330 (LA353_21325) elbB 4426499..4427152 (-) 654 WP_047051241.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=606666 LA353_RS21300 WP_003861864.1 4422251..4423684(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=606666 LA353_RS21300 WP_003861864.1 4422251..4423684(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. oharae strain FDAARGOS_1533]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGTAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTAGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
AATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCCCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATATATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48