Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD56_RS19730 Genome accession   NZ_CP100901
Coordinates   4071848..4072288 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain ET826     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066848..4077288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD56_RS19710 (NMD56_19595) aroP 4067846..4069219 (+) 1374 WP_001331235.1 aromatic amino acid transporter AroP -
  NMD56_RS19715 (NMD56_19600) ampE 4069262..4070116 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD56_RS19720 (NMD56_19605) ampD 4070113..4070664 (-) 552 WP_001402016.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD56_RS19725 (NMD56_19610) nadC 4070752..4071645 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD56_RS19730 (NMD56_19615) pilA 4071848..4072288 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NMD56_RS19735 (NMD56_19620) pilB 4072298..4073683 (+) 1386 WP_001402015.1 type II secretion system protein GspE Machinery gene
  NMD56_RS19740 (NMD56_19625) hofC 4073673..4074875 (+) 1203 WP_000157236.1 protein transport protein HofC -
  NMD56_RS19745 (NMD56_19630) guaC 4074910..4075953 (-) 1044 WP_001217338.1 GMP reductase -
  NMD56_RS19750 (NMD56_19635) - 4076109..4076153 (-) 45 WP_120795372.1 protein YacM -
  NMD56_RS19755 (NMD56_19640) coaE 4076178..4076798 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=606576 NMD56_RS19730 WP_000360895.1 4071848..4072288(+) (pilA) [Escherichia coli strain ET826]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606576 NMD56_RS19730 WP_000360895.1 4071848..4072288(+) (pilA) [Escherichia coli strain ET826]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363