Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD57_RS20050 Genome accession   NZ_CP100895
Coordinates   4004244..4004684 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET846     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3999244..4009684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD57_RS20030 (NMD57_19895) aroP 4000242..4001615 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD57_RS20035 (NMD57_19900) ampE 4001658..4002512 (-) 855 WP_115799366.1 beta-lactamase regulator AmpE -
  NMD57_RS20040 (NMD57_19905) ampD 4002509..4003060 (-) 552 WP_032083627.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD57_RS20045 (NMD57_19910) nadC 4003148..4004041 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD57_RS20050 (NMD57_19915) pilA 4004244..4004684 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD57_RS20055 (NMD57_19920) pilB 4004694..4006079 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD57_RS20060 (NMD57_19925) hofC 4006069..4007271 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD57_RS20065 (NMD57_19930) guaC 4007306..4008349 (-) 1044 WP_001217338.1 GMP reductase -
  NMD57_RS20070 (NMD57_19935) - 4008505..4008549 (-) 45 WP_120795372.1 protein YacM -
  NMD57_RS20075 (NMD57_19940) coaE 4008574..4009194 (+) 621 WP_001269532.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=606490 NMD57_RS20050 WP_000360904.1 4004244..4004684(+) (pilA) [Escherichia coli strain ET846]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606490 NMD57_RS20050 WP_000360904.1 4004244..4004684(+) (pilA) [Escherichia coli strain ET846]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363