Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD58_RS18795 Genome accession   NZ_CP100890
Coordinates   3809929..3810369 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET881     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3804929..3815369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD58_RS18775 (NMD58_18645) aroP 3805927..3807300 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD58_RS18780 (NMD58_18650) ampE 3807343..3808197 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NMD58_RS18785 (NMD58_18655) ampD 3808194..3808745 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD58_RS18790 (NMD58_18660) nadC 3808833..3809726 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD58_RS18795 (NMD58_18665) pilA 3809929..3810369 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD58_RS18800 (NMD58_18670) pilB 3810379..3811764 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD58_RS18805 (NMD58_18675) hofC 3811754..3812956 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD58_RS18810 (NMD58_18680) guaC 3812991..3814034 (-) 1044 WP_001217338.1 GMP reductase -
  NMD58_RS18815 (NMD58_18685) - 3814190..3814234 (-) 45 WP_120795372.1 protein YacM -
  NMD58_RS18820 (NMD58_18690) coaE 3814259..3814879 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=606407 NMD58_RS18795 WP_000360904.1 3809929..3810369(+) (pilA) [Escherichia coli strain ET881]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606407 NMD58_RS18795 WP_000360904.1 3809929..3810369(+) (pilA) [Escherichia coli strain ET881]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363