Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LAZ82_RS20040 Genome accession   NZ_CP083589
Coordinates   4455974..4457464 (+) Length   496 a.a.
NCBI ID   WP_102663446.1    Uniprot ID   -
Organism   Herbaspirillum frisingense strain AU14559     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4450974..4462464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAZ82_RS20015 (LAZ82_20025) - 4452301..4453329 (+) 1029 WP_006461690.1 SIS domain-containing protein -
  LAZ82_RS20020 (LAZ82_20030) - 4453329..4453883 (+) 555 WP_006461689.1 KdsC family phosphatase -
  LAZ82_RS20025 (LAZ82_20035) lptC 4453880..4454488 (+) 609 WP_102663444.1 LPS export ABC transporter periplasmic protein LptC -
  LAZ82_RS20030 (LAZ82_20040) lptA 4454540..4455163 (+) 624 WP_039874062.1 lipopolysaccharide transport periplasmic protein LptA -
  LAZ82_RS20035 (LAZ82_20045) lptB 4455166..4455906 (+) 741 WP_006461686.1 LPS export ABC transporter ATP-binding protein -
  LAZ82_RS20040 (LAZ82_20050) rpoN/rpoN1 4455974..4457464 (+) 1491 WP_102663446.1 RNA polymerase factor sigma-54 Machinery gene
  LAZ82_RS20045 (LAZ82_20055) hpf 4457623..4457985 (+) 363 WP_006461684.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LAZ82_RS20050 (LAZ82_20060) grxD 4458360..4458671 (+) 312 WP_006461683.1 Grx4 family monothiol glutaredoxin -
  LAZ82_RS20055 (LAZ82_20065) - 4458707..4459309 (+) 603 WP_234294608.1 UbiX family flavin prenyltransferase -
  LAZ82_RS20060 (LAZ82_20070) - 4459388..4460146 (-) 759 WP_234294609.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 55088.57 Da        Isoelectric Point: 5.1177

>NTDB_id=606392 LAZ82_RS20040 WP_102663446.1 4455974..4457464(+) (rpoN/rpoN1) [Herbaspirillum frisingense strain AU14559]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQILSDNPLLERLDDALDNSVRLLADGAVSSAPSTGVAEPAGD
SGNEAAPAATDGEASFDYQDNSPESGVSGESDWSFDDVARSGKSPDDDDARPQLEAHELTLREHLLEQIRVTARTHRDRA
LLELLTDALDESGYLEEPLEDILARLPEELGVDLEELSISLKLLQSLDPAGVGARSAGECLALQIRRLPKVPFVTRKLAL
RIVEDYLPLFAQRDFNKIKKALDCDDEDLREAQTVIRQCRPHPGAEFARDASDYVVPDVIVKQTKNGWQVMLNHEVMPKL
RVNAMYASALKQAKGEGSLSSQLQEAKWLIKNMRQRFDTILRVAQAIVERQRNFFSHGAVAMRPLVLREIADTLGLHEST
ISRVTTQKYMLTPHGMFELKYFFGSHVATETGGEASSTAIRALIKQLIGAENPQTPLSDSKIADMLAEQGMVVARRTVAK
YREVLKIPPVNLRKSL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=606392 LAZ82_RS20040 WP_102663446.1 4455974..4457464(+) (rpoN/rpoN1) [Herbaspirillum frisingense strain AU14559]
ATGAAACAGTCGCTCCAGTTACGTACCTCGCAGCACCTCGCATTGACGCCGCAACTGCAGCAGTCGATCAGGCTGCTGCA
ACTGTCGACGCTGGAACTGCACCAGGAACTGGAACAGATCCTGTCCGACAATCCCCTGCTCGAACGCCTGGACGACGCAC
TCGACAATTCGGTGCGCCTGTTGGCCGATGGTGCGGTCTCTTCGGCGCCCTCCACCGGAGTGGCCGAGCCCGCTGGCGAC
AGCGGCAACGAAGCAGCTCCCGCCGCTACCGATGGCGAAGCCAGTTTCGACTATCAGGACAACAGTCCCGAGAGCGGTGT
CAGTGGTGAATCCGACTGGAGCTTCGACGACGTGGCACGCAGTGGCAAATCGCCCGACGACGACGATGCCCGCCCGCAAC
TGGAAGCGCATGAGCTGACCCTGCGCGAACACCTGCTGGAACAGATCCGGGTCACCGCCCGCACGCACCGCGACCGTGCG
CTGCTGGAACTGCTCACCGATGCGCTGGACGAAAGTGGTTACCTCGAAGAACCGCTGGAAGACATCCTGGCGCGCCTGCC
GGAAGAGCTGGGCGTTGACCTGGAAGAACTGTCGATCTCGCTGAAGCTGCTGCAGAGCCTGGACCCGGCCGGAGTGGGTG
CGCGCAGTGCCGGTGAATGTCTGGCACTGCAGATCCGCCGCCTGCCCAAGGTGCCCTTCGTCACCCGCAAGCTGGCCTTG
CGGATTGTCGAGGATTACCTGCCGCTGTTCGCACAGCGCGATTTCAACAAGATCAAGAAGGCCCTCGATTGCGATGACGA
GGACCTGCGCGAAGCACAGACGGTGATCCGTCAGTGCCGTCCGCATCCGGGTGCCGAATTCGCGCGCGATGCTTCGGACT
ATGTGGTGCCGGACGTGATTGTCAAACAGACAAAGAACGGCTGGCAGGTCATGCTCAACCATGAGGTCATGCCCAAGCTG
CGCGTCAATGCAATGTATGCGAGTGCGCTCAAGCAGGCCAAGGGCGAAGGATCGCTGTCTTCCCAGTTGCAGGAAGCCAA
GTGGCTCATCAAGAACATGCGGCAACGTTTCGATACAATTTTGCGAGTCGCGCAGGCTATTGTCGAACGTCAAAGAAACT
TCTTTTCACATGGCGCAGTCGCCATGCGGCCCCTTGTGCTGCGTGAAATTGCTGATACACTGGGTCTACACGAGAGCACA
ATCTCTCGCGTGACAACTCAGAAATACATGCTCACGCCGCACGGCATGTTCGAGTTGAAGTACTTCTTCGGTAGCCACGT
CGCGACTGAAACCGGAGGCGAAGCCTCCTCCACCGCGATACGGGCGCTGATCAAACAGTTGATAGGAGCCGAAAACCCGC
AGACCCCATTATCCGATAGCAAGATTGCGGATATGCTGGCAGAACAAGGCATGGTGGTCGCGCGACGCACAGTCGCCAAA
TACCGCGAAGTGTTGAAGATTCCGCCAGTCAATCTCCGCAAGTCCCTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(339-494)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

59.449

100

0.609