Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   K9N56_RS15020 Genome accession   NZ_CP083580
Coordinates   2953330..2954397 (-) Length   355 a.a.
NCBI ID   WP_006637540.1    Uniprot ID   -
Organism   Bacillus sp. PM8313     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2948330..2959397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N56_RS14975 (K9N56_14975) tapA 2948758..2949504 (-) 747 WP_006637531.1 amyloid fiber anchoring/assembly protein TapA -
  K9N56_RS14980 (K9N56_14980) - 2949767..2950087 (+) 321 WP_006637532.1 DUF3889 domain-containing protein -
  K9N56_RS14985 (K9N56_14985) - 2950181..2950366 (-) 186 WP_006637533.1 YqzE family protein -
  K9N56_RS14990 (K9N56_14990) comGG 2950446..2950769 (-) 324 WP_224254418.1 competence type IV pilus minor pilin ComGG -
  K9N56_RS14995 (K9N56_14995) comGF 2950824..2951315 (-) 492 WP_224254419.1 competence type IV pilus minor pilin ComGF -
  K9N56_RS15000 (K9N56_15000) comGE 2951224..2951571 (-) 348 WP_006637536.1 competence type IV pilus minor pilin ComGE -
  K9N56_RS15005 (K9N56_15005) comGD 2951555..2951998 (-) 444 WP_006637537.1 competence type IV pilus minor pilin ComGD -
  K9N56_RS15010 (K9N56_15010) comGC 2951998..2952291 (-) 294 WP_006637538.1 competence type IV pilus major pilin ComGC Machinery gene
  K9N56_RS15015 (K9N56_15015) comGB 2952306..2953343 (-) 1038 WP_006637539.1 competence type IV pilus assembly protein ComGB Machinery gene
  K9N56_RS15020 (K9N56_15020) comGA 2953330..2954397 (-) 1068 WP_006637540.1 competence type IV pilus ATPase ComGA Machinery gene
  K9N56_RS15025 (K9N56_15025) - 2954536..2955393 (-) 858 WP_006637541.1 STAS domain-containing protein -
  K9N56_RS15035 (K9N56_15035) - 2955612..2955992 (-) 381 WP_006637542.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  K9N56_RS15040 (K9N56_15040) - 2956205..2956450 (+) 246 WP_006637543.1 DUF2626 domain-containing protein -
  K9N56_RS15045 (K9N56_15045) - 2956500..2957138 (-) 639 WP_006637544.1 MBL fold metallo-hydrolase -
  K9N56_RS15050 (K9N56_15050) - 2957279..2957452 (+) 174 WP_006637545.1 DUF2759 domain-containing protein -
  K9N56_RS15055 (K9N56_15055) - 2957510..2957824 (-) 315 WP_006637546.1 MTH1187 family thiamine-binding protein -
  K9N56_RS15060 (K9N56_15060) - 2957839..2958948 (-) 1110 WP_029419374.1 hypothetical protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39821.31 Da        Isoelectric Point: 9.4732

>NTDB_id=606303 K9N56_RS15020 WP_006637540.1 2953330..2954397(-) (comGA) [Bacillus sp. PM8313]
MQAIEPLSGRVIEEACRMRASDIHIVPCKKEAIIRFRIDGELIQKDRLTRLECSRLISHFKFLSSMDIGERRQPQSGALT
LQVNNKPVHLRMSTLPTVYDESLVIRVLPQASAPPLRSLSLFPDATSKLLSFLKHSHGLMIFTGPTGSGKTTTLYSLIEY
AKQHFNRNIITLEDPVESRSEHVLQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAKTAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVTMTEIEQTLVAVSAQRLVNLVCPFCGEQCSFYCRMAREVRRASIFELLYGKSLNLCIKEAKGAY
VNNRFDTLRKLIRKGIALGYVPAGSYERWVHHEAD

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=606303 K9N56_RS15020 WP_006637540.1 2953330..2954397(-) (comGA) [Bacillus sp. PM8313]
TTGCAAGCGATTGAACCATTAAGCGGGAGAGTAATCGAAGAGGCATGCAGAATGAGAGCATCTGACATTCATATTGTTCC
GTGTAAAAAAGAGGCGATTATCCGTTTTAGAATCGACGGTGAATTGATTCAAAAGGACAGGCTGACGAGGCTTGAGTGCT
CAAGGCTGATTTCCCACTTTAAATTTCTTTCTTCAATGGATATCGGAGAACGGAGACAGCCGCAAAGCGGTGCTTTAACC
CTTCAAGTGAACAATAAGCCTGTTCATTTAAGAATGTCGACTTTGCCCACTGTATACGATGAAAGCTTGGTGATCCGCGT
TTTGCCGCAAGCAAGTGCCCCGCCGCTCAGAAGCCTGTCATTGTTTCCGGATGCAACGTCGAAGCTGCTGTCTTTTCTGA
AGCATTCACACGGCCTGATGATCTTCACCGGCCCTACAGGTTCGGGAAAAACGACAACTCTGTATTCGCTAATCGAATAT
GCAAAACAGCATTTTAACCGCAATATTATTACCCTGGAGGATCCGGTGGAATCCAGAAGCGAGCATGTTCTTCAAGTACA
GGTAAATGAGAAGGCGGGTATGACATATTCTGCCGGCTTAAAGGCTGTTCTCCGCCATGATCCGGACATGATCATTCTGG
GGGAAATCCGCGATGCAGAAACAGCCAAAACCGCGGTCAGAGCGGCGCTGACGGGTCATCTTGTATTATCGAGCATGCAC
GCGAAAAACGCAAAAGGCGCGATATACAGACTGCTTGAATTCGGCGTCACAATGACAGAAATTGAACAGACGCTGGTTGC
TGTAAGCGCGCAACGACTCGTCAATCTTGTCTGTCCATTTTGCGGAGAGCAGTGTTCTTTTTATTGCAGAATGGCGAGGG
AAGTCAGAAGAGCAAGCATTTTTGAGCTTCTGTATGGAAAGAGCCTGAATCTTTGTATAAAAGAAGCTAAAGGCGCATAT
GTAAACAACCGCTTCGACACATTGAGAAAATTGATCCGCAAAGGGATAGCGCTCGGTTATGTGCCGGCCGGATCTTATGA
ACGCTGGGTGCATCATGAAGCCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.978

100

0.682