Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABEDC_RS13145 Genome accession   NZ_CP083571
Coordinates   2718461..2719261 (-) Length   266 a.a.
NCBI ID   WP_005103248.1    Uniprot ID   N9QCA1
Organism   Acinetobacter lwoffii strain ED9-5a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2713461..2724261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEDC_RS13125 (ABEDC_2734) - 2714506..2715204 (-) 699 WP_247143816.1 tetratricopeptide repeat protein -
  ABEDC_RS13130 (ABEDC_2735) hisS 2715235..2716530 (-) 1296 WP_374952253.1 histidine--tRNA ligase -
  ABEDC_RS13135 (ABEDC_2736) ispG 2716534..2717655 (-) 1122 WP_374952254.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABEDC_RS13140 (ABEDC_2737) - 2717679..2718470 (-) 792 WP_004278356.1 DUF4115 domain-containing protein -
  ABEDC_RS13145 (ABEDC_2738) pilF 2718461..2719261 (-) 801 WP_005103248.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABEDC_RS13150 (ABEDC_2739) rlmN 2719276..2720511 (-) 1236 WP_004647461.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABEDC_RS13155 (ABEDC_2740) ndk 2720634..2721065 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ABEDC_RS13160 (ABEDC_2741) iscX 2721176..2721376 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  ABEDC_RS13165 (ABEDC_2742) - 2721464..2722015 (-) 552 WP_004278361.1 phosphatase PAP2 family protein -
  ABEDC_RS13170 (ABEDC_2743) - 2722036..2723316 (-) 1281 WP_004647459.1 glycosyltransferase family 1 protein -
  ABEDC_RS13175 (ABEDC_2744) - 2723585..2724001 (-) 417 WP_004647457.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=606242 ABEDC_RS13145 WP_005103248.1 2718461..2719261(-) (pilF) [Acinetobacter lwoffii strain ED9-5a]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=606242 ABEDC_RS13145 WP_005103248.1 2718461..2719261(-) (pilF) [Acinetobacter lwoffii strain ED9-5a]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGTGCATTGGAAAATATGGGACGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTAGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTTGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGGATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QCA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.549

100

0.695