Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N134_RS01560 Genome accession   NC_021872
Coordinates   383362..384735 (+) Length   457 a.a.
NCBI ID   WP_020842886.1    Uniprot ID   S5NMP9
Organism   Limosilactobacillus reuteri TD1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 378362..389735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N134_RS01530 (N134_01560) - 378512..379261 (+) 750 WP_020842882.1 amino acid ABC transporter ATP-binding protein -
  N134_RS01535 (N134_01565) - 379277..380068 (+) 792 WP_019252998.1 transporter substrate-binding domain-containing protein -
  N134_RS01540 (N134_01570) - 380301..381641 (-) 1341 WP_020842883.1 aminopeptidase C -
  N134_RS01545 (N134_01575) rpiA 381670..382353 (-) 684 WP_020842884.1 ribose-5-phosphate isomerase RpiA -
  N134_RS01550 (N134_01580) - 382365..382661 (-) 297 WP_003666282.1 GNAT family N-acetyltransferase -
  N134_RS01555 (N134_01585) - 382800..383336 (+) 537 WP_020842885.1 dUTP diphosphatase -
  N134_RS01560 (N134_01590) radA 383362..384735 (+) 1374 WP_020842886.1 DNA repair protein RadA Machinery gene
  N134_RS01565 (N134_01595) - 384754..385905 (+) 1152 WP_020842887.1 PIN/TRAM domain-containing protein -
  N134_RS01570 (N134_01600) cysS 386280..387692 (+) 1413 WP_042229207.1 cysteine--tRNA ligase -
  N134_RS01575 (N134_01605) - 387704..388117 (+) 414 WP_020842889.1 Mini-ribonuclease 3 -
  N134_RS01580 (N134_01610) rlmB 388107..388856 (+) 750 WP_020842890.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  N134_RS10210 (N134_01615) rpmG 388922..389071 (+) 150 WP_003666293.1 50S ribosomal protein L33 -
  N134_RS01585 (N134_01620) secE 389083..389259 (+) 177 WP_003666294.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49919.17 Da        Isoelectric Point: 8.1257

>NTDB_id=60620 N134_RS01560 WP_020842886.1 383362..384735(+) (radA) [Limosilactobacillus reuteri TD1]
MAKVRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVEASSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLALAVSIASSYRDKGTQPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVVGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=60620 N134_RS01560 WP_020842886.1 383362..384735(+) (radA) [Limosilactobacillus reuteri TD1]
ATGGCAAAAGTTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGTCGGTGTCCAAACTG
TGGTGAGTGGAATACTTTAGTAGAAGAACAGGTAGAAGCCAGTTCTGCACCAACAAAAAAAGCAACCACCACCTTAACTG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAGACTCCCCGCGTTAAAACAAAATTGAACGAGTTA
AATCGCGTTTTAGGTGGGGGAATCGTTCCAGGATCACTAATTCTAATTGGTGGGGATCCCGGAATCGGGAAATCCACCCT
CCTTTTACAAGTTTCTGGACAATTGAGCGATGAGCATCACCGAGTTTTATATGTGTCTGGAGAAGAAAGCGGGACACAGA
TTAAAATGCGGGCGGAACGGTTGAAGGTTGCTGGAGATGACTTTTATGTGTATCCAGAAACAAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTCGTTATTGACTCCGTGCAAACGATGCAGGCAACTGATGTAAGTTCAGC
GATTGGTAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAGATTGCTAAAAGTAATAACATTACGATTTTTG
TTGTTGGTCATGTGACAAAGGGTGGTGCAATCGCCGGTCCAAAGATTTTAGAGCACATGGTTGATACGGTTTTGTATTTT
GAAGGAGATCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACCAACGAGCTTGGTATTTT
CGAGATGCATACAAATGGACTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACTG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACGCGACCGATTTTAGTGGAAATTCAGGCGCTGGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACTGCTACCGGTTTGAGTCGAAACCGAGTATCATTGATAATGGCAGTATTAGAGAAACGTGCTAA
CTTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAATTAGATGAGCCAGCGATTGATTTAGCAC
TTGCGGTAAGCATTGCCTCTAGTTACCGAGATAAAGGTACCCAACCAACTGACGCATTTGTCGGTGAAGTAGGTTTAACT
GGGGAAATTCGTCGGGTAAACCGCATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGCTTTAAACGGATCTTTATTCC
CAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAGTCGGTGTCTCAACCCTGAAAGAAGCGTTATATT
TAGCGTTAGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5NMP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.372

98.906

0.656
  radA Streptococcus mitis NCTC 12261

66.372

98.906

0.656
  radA Streptococcus pneumoniae Rx1

66.15

98.906

0.654
  radA Streptococcus pneumoniae D39

66.15

98.906

0.654
  radA Streptococcus pneumoniae R6

66.15

98.906

0.654
  radA Streptococcus pneumoniae TIGR4

66.15

98.906

0.654
  radA Bacillus subtilis subsp. subtilis str. 168

62.582

100

0.626

Multiple sequence alignment