Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD61_RS19835 Genome accession   NZ_CP100876
Coordinates   3991717..3992157 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET979     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3986717..3997157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD61_RS19815 (NMD61_19680) aroP 3987715..3989088 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD61_RS19820 (NMD61_19685) ampE 3989131..3989985 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD61_RS19825 (NMD61_19690) ampD 3989982..3990533 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD61_RS19830 (NMD61_19695) nadC 3990621..3991514 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD61_RS19835 (NMD61_19700) pilA 3991717..3992157 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD61_RS19840 (NMD61_19705) pilB 3992167..3993552 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NMD61_RS19845 (NMD61_19710) hofC 3993542..3994744 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NMD61_RS19850 (NMD61_19715) guaC 3994779..3995822 (-) 1044 WP_001217338.1 GMP reductase -
  NMD61_RS19855 (NMD61_19720) - 3995978..3996022 (-) 45 WP_120795372.1 protein YacM -
  NMD61_RS19860 (NMD61_19725) coaE 3996047..3996667 (+) 621 WP_016232452.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=606162 NMD61_RS19835 WP_000360904.1 3991717..3992157(+) (pilA) [Escherichia coli strain ET979]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=606162 NMD61_RS19835 WP_000360904.1 3991717..3992157(+) (pilA) [Escherichia coli strain ET979]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363