Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LAG74_RS01670 Genome accession   NZ_CP083461
Coordinates   346627..348066 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain SW3814     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341627..353066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAG74_RS01640 (LAG74_01645) mlaF 342204..343004 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  LAG74_RS01645 (LAG74_01650) - 343212..344207 (+) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  LAG74_RS01650 (LAG74_01655) kdsC 344207..344761 (+) 555 WP_047438734.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  LAG74_RS01655 (LAG74_01660) lptC 344758..345318 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  LAG74_RS01660 (LAG74_01665) lptA 345305..345832 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  LAG74_RS01665 (LAG74_01670) lptB 345836..346561 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  LAG74_RS01670 (LAG74_01675) rpoN/rpoN1 346627..348066 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  LAG74_RS01675 (LAG74_01680) hpf 348087..348374 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  LAG74_RS01680 (LAG74_01685) ptsN 348377..348823 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LAG74_RS01685 (LAG74_01690) rapZ 348869..349735 (+) 867 WP_005336320.1 RNase adapter RapZ -
  LAG74_RS01690 (LAG74_01695) npr 349756..350028 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  LAG74_RS01695 (LAG74_01700) - 350046..351950 (-) 1905 WP_224969324.1 PRD domain-containing protein -
  LAG74_RS01700 (LAG74_01705) - 352058..352798 (-) 741 WP_224969325.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=606004 LAG74_RS01670 WP_005336331.1 346627..348066(+) (rpoN/rpoN1) [Aeromonas veronii strain SW3814]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=606004 LAG74_RS01670 WP_005336331.1 346627..348066(+) (rpoN/rpoN1) [Aeromonas veronii strain SW3814]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCATGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGTTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATTCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCACGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAATGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGCCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491