Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NLZ06_RS18285 Genome accession   NZ_CP100544
Coordinates   3814199..3814639 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain LH09-a     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3809199..3819639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLZ06_RS18265 (NLZ06_18265) aroP 3810197..3811570 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  NLZ06_RS18270 (NLZ06_18270) ampE 3811613..3812467 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NLZ06_RS18275 (NLZ06_18275) ampD 3812464..3813015 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NLZ06_RS18280 (NLZ06_18280) nadC 3813103..3813996 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NLZ06_RS18285 (NLZ06_18285) pilA 3814199..3814639 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NLZ06_RS18290 (NLZ06_18290) pilB 3814649..3816034 (+) 1386 WP_001025174.1 type II secretion system protein GspE Machinery gene
  NLZ06_RS18295 (NLZ06_18295) hofC 3816024..3817226 (+) 1203 WP_000157236.1 protein transport protein HofC -
  NLZ06_RS18300 (NLZ06_18300) guaC 3817261..3818304 (-) 1044 WP_001217338.1 GMP reductase -
  NLZ06_RS18305 (NLZ06_18305) - 3818460..3818504 (-) 45 WP_120795372.1 protein YacM -
  NLZ06_RS18310 (NLZ06_18310) coaE 3818529..3819149 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=605884 NLZ06_RS18285 WP_000360904.1 3814199..3814639(+) (pilA) [Escherichia coli strain LH09-a]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=605884 NLZ06_RS18285 WP_000360904.1 3814199..3814639(+) (pilA) [Escherichia coli strain LH09-a]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363