Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HK81_RS23205 Genome accession   NC_021848
Coordinates   3302164..3303180 (+) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus O1:K33 str. CDC_K4557     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3297164..3308180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HK81_RS23190 (M636_23700) - 3297588..3298316 (-) 729 WP_015297397.1 redoxin family protein -
  HK81_RS23195 (M636_23705) oxyR 3298487..3299395 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  HK81_RS23200 (M636_23710) - 3299451..3302021 (-) 2571 WP_020904548.1 penicillin-binding protein 1A -
  HK81_RS23205 (M636_23715) pilM 3302164..3303180 (+) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene
  HK81_RS23210 (M636_23720) pilN 3303164..3303742 (+) 579 WP_020904549.1 PilN domain-containing protein Machinery gene
  HK81_RS23215 (M636_23725) pilO 3303735..3304328 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  HK81_RS23220 (M636_23730) pilP 3304318..3304833 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  HK81_RS23225 (M636_23735) pilQ 3304865..3306613 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  HK81_RS23230 (M636_23740) aroK 3306802..3307320 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=60588 HK81_RS23205 WP_005496568.1 3302164..3303180(+) (pilM) [Vibrio parahaemolyticus O1:K33 str. CDC_K4557]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=60588 HK81_RS23205 WP_005496568.1 3302164..3303180(+) (pilM) [Vibrio parahaemolyticus O1:K33 str. CDC_K4557]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GCTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTAGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment