Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K6V71_RS19545 Genome accession   NZ_CP083438
Coordinates   2256169..2257647 (-) Length   492 a.a.
NCBI ID   WP_053821037.1    Uniprot ID   A0A849BDH7
Organism   Cupriavidus gilardii strain NOV2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2251169..2262647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6V71_RS19515 (K6V71_19420) - 2251564..2252217 (-) 654 WP_053821032.1 LON peptidase substrate-binding domain-containing protein -
  K6V71_RS19520 (K6V71_19425) rapZ 2252302..2253183 (-) 882 WP_053821033.1 RNase adapter RapZ -
  K6V71_RS19525 (K6V71_19430) - 2253420..2253710 (-) 291 WP_053821034.1 PsiF family protein -
  K6V71_RS19530 (K6V71_19435) hprK 2253887..2254858 (-) 972 WP_006576781.1 HPr(Ser) kinase/phosphatase -
  K6V71_RS19535 (K6V71_19440) ptsN 2254931..2255386 (-) 456 WP_053821035.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K6V71_RS19540 (K6V71_19445) hpf 2255766..2256119 (-) 354 WP_053821036.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K6V71_RS19545 (K6V71_19450) rpoN/rpoN1 2256169..2257647 (-) 1479 WP_053821037.1 RNA polymerase factor sigma-54 Machinery gene
  K6V71_RS19550 (K6V71_19455) lptB 2257839..2258663 (-) 825 WP_006579161.1 LPS export ABC transporter ATP-binding protein -
  K6V71_RS19555 (K6V71_19460) lptA 2258746..2259375 (-) 630 WP_053822969.1 lipopolysaccharide transport periplasmic protein LptA -
  K6V71_RS19560 (K6V71_19465) lptC 2259456..2260082 (-) 627 WP_053821038.1 LPS export ABC transporter periplasmic protein LptC -
  K6V71_RS19565 (K6V71_19470) - 2260091..2261074 (-) 984 WP_053822970.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 492 a.a.        Molecular weight: 54891.27 Da        Isoelectric Point: 5.0336

>NTDB_id=605836 K6V71_RS19545 WP_053821037.1 2256169..2257647(-) (rpoN/rpoN1) [Cupriavidus gilardii strain NOV2-1]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQTATTTSPAEPA
APADSHADGTAAADEGYADGAGDDYGGDWSLDDFARRPQSDEDEKTPLQLRDAEPTLREYLMEQLTPLKISTRDKGLAIF
LIESLDDDGYLSASLEEILSELPEELEFELDEVHAMLTLLQSFDPPGVGARNAAECLSLQLRRIEHPQRELAMTIVRHHL
ELLAARDYTRLKKALQVDDMQLKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGHNWIAQLNPDVMPRLRINDMYA
QILRGAKGDTGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRV
TTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYREA
LKIPAVNLRKSL

Nucleotide


Download         Length: 1479 bp        

>NTDB_id=605836 K6V71_RS19545 WP_053821037.1 2256169..2257647(-) (rpoN/rpoN1) [Cupriavidus gilardii strain NOV2-1]
ATGAAACCGTCGCTACAGCTACGCCTCTCCCAGCATCTGGCCCTGACGCCGCAGCTGCAGCAGTCCATCCGGCTGTTGCA
GTTGTCCACGCTGGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAATCCGCTGCTCGAACGCGAAAACGACTGGA
TCGAAAGCCCGCTGCGCGTTGCCGCCGACGGGTCGGTCAATGTGCAGACCGCCACCACGACCTCGCCCGCCGAACCGGCG
GCGCCCGCGGACTCGCACGCGGACGGCACCGCCGCAGCCGACGAAGGCTATGCCGACGGCGCGGGCGACGATTATGGCGG
CGACTGGAGCCTGGACGACTTCGCCCGCCGTCCGCAAAGCGATGAGGACGAGAAAACACCGCTGCAATTGCGCGATGCCG
AGCCCACGCTGCGCGAATATCTGATGGAGCAATTGACGCCGCTGAAAATCTCGACGCGCGACAAGGGCCTGGCGATTTTC
CTGATCGAATCGCTCGACGACGACGGTTATCTGAGCGCGTCGCTCGAGGAAATCCTGTCCGAATTGCCGGAAGAGCTCGA
ATTCGAACTCGACGAAGTGCATGCGATGCTGACGCTGCTGCAAAGCTTCGATCCGCCCGGGGTCGGCGCACGCAACGCAG
CAGAGTGCCTGTCGCTGCAACTGCGCCGCATCGAACATCCGCAGCGCGAGCTGGCGATGACCATCGTCCGCCATCACCTC
GAACTGCTGGCCGCGCGCGACTACACACGGCTGAAGAAGGCGCTGCAGGTCGACGACATGCAGCTGAAGGCCGCGCACGA
GTTGATCCGCTCGCTCGCGCCGTATCCCGGCCACGCCTACAGCCGGCCGGAAGCGGACTTCGTGGTGCCCGATGTATTCG
TGCGCAAGAGCGGCCACAACTGGATCGCCCAGCTCAATCCCGATGTGATGCCGCGGCTACGCATCAACGACATGTATGCG
CAGATCTTGCGCGGTGCGAAAGGCGATACCGGCGCCGCGGGATTGCAGCAGAAACTGCAGGAGGCGCGCTGGCTCATCAA
AAATATCCAGCAGCGTTTCGACACCATTTTGCGCGTCGCACAAGCCATCGTGGAGCGGCAAAAGAATTTCTTTACGCACG
GCGAAATTGCGATGCGCCCCTTGGTTTTGAGGGAGATAGCCGATACACTGGGTCTACACGAGTCGACTATTTCTCGTGTG
ACGACCAACAAGTACATGGCCACCCCGATGGGGACATTCGAGCTGAAATATTTTTTCGGCAGCCATGTATCGACCGAGAC
CGGCGGTGCCGCCTCTTCCACCGCAATCCGTGCGCTGATCAAGCAGCTGATAGGAGCCGAAGACCCCAGAAACCCGTTGT
CCGACAGCCGGATTGCCGAACTGCTGGGGGAACAAGGCTTCGTCGTGGCGCGACGTACCGTCGCCAAATATCGTGAAGCC
TTGAAGATCCCGGCCGTGAACCTTCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-319)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-490)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A849BDH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.958

100

0.801