Detailed information NTG0605801

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K9864_RS13670
• Genome accession: NZ_CP083436
• Coordinates: 2742042..2742656 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain HC-8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737042..2747656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K9864_RS13640 (K9864_13545)	rpsT	2737345..2737611 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
K9864_RS13645 (K9864_13550)	holA	2737628..2738669 (-)	1042	Protein_2646	DNA polymerase III subunit delta	-
K9864_RS13650 (K9864_13555)	-	2738709..2738860 (-)	152	Protein_2647	hypothetical protein	-
K9864_RS13655 (K9864_13560)	-	2738901..2739035 (+)	135	WP_003152870.1	YqzM family protein	-
K9864_RS13660 (K9864_13565)	comEC	2739054..2741405 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K9864_RS13665 (K9864_13570)	-	2741406..2741975 (-)	570	WP_003152868.1	ComE operon protein 2	-
K9864_RS13670 (K9864_13575)	comEA	2742042..2742656 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
K9864_RS13675 (K9864_13580)	comER	2742715..2743536 (+)	822	WP_014418420.1	late competence protein ComER	-
K9864_RS13680 (K9864_13585)	-	2743605..2744342 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
K9864_RS13685 (K9864_13590)	rsfS	2744339..2744695 (-)	357	WP_007408260.1	ribosome silencing factor	-
K9864_RS13690 (K9864_13595)	yqeK	2744713..2745273 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K9864_RS13695 (K9864_13600)	-	2745263..2745832 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
K9864_RS13700 (K9864_13605)	yhbY	2745843..2746133 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
K9864_RS13705 (K9864_13610)	aroE	2746127..2746969 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=605801 K9864_RS13670 WP_014418419.1 2742042..2742656(-) (comEA) [Bacillus velezensis strain HC-8]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=605801 K9864_RS13670 WP_014418419.1 2742042..2742656(-) (comEA) [Bacillus velezensis strain HC-8]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363