Detailed information NTG0605107

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: K8Z53_RS16950
• Genome accession: NZ_CP083238
• Coordinates: 3218589..3222281 (+)
• Length: 1230 a.a.
• NCBI ID: WP_011197743.1
• Uniprot ID: Q65LJ9
• Organism: Bacillus licheniformis strain 2709
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3213589..3227281

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8Z53_RS16940 (K8Z53_16760)	modB	3214336..3214992 (+)	657	WP_009328900.1	molybdate ABC transporter permease subunit	-
K8Z53_RS16945 (K8Z53_16765)	addB	3215102..3218602 (+)	3501	WP_009328899.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
K8Z53_RS16950 (K8Z53_16770)	addA	3218589..3222281 (+)	3693	WP_011197743.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
K8Z53_RS16955 (K8Z53_16775)	sbcD	3222346..3223527 (+)	1182	WP_073426309.1	exonuclease subunit SbcD	-
K8Z53_RS16960 (K8Z53_16780)	sbcC	3223524..3226916 (+)	3393	WP_061576187.1	exonuclease subunit SbcC	-
K8Z53_RS16965 (K8Z53_16785)	-	3226961..3227179 (-)	219	WP_142782039.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141090.95 Da
• Isoelectric Point: 5.5198

>NTDB_id=605107 K8Z53_RS16950 WP_011197743.1 3218589..3222281(+) (addA) [Bacillus licheniformis strain 2709]
MEISKPKGSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQERPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKGNPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHSLAELYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQDDFSALHELVPTVSFQRLKPCKGDEYDPRLVDEAADLRNSAKKQLEKLK
SDYFSRTPEQHLESLREMKPVIQTLVQLVLEYGRRFAEAKKEKAIVDFSDLEHDCLAILSVKNPAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPPNEATKTELLLIETPSGAPGEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDGLLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRTQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPPVIDEIREHPARFQVSWLSAADLQAE
AVDQAGEERHDRLVQIQMGHAIEGAFEYEQQVRERLAWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPSQEAL
LFKRPSFMMAKGLTAAERGTAMHTVMQHIPLTRTPEKNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLFG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=605107 K8Z53_RS16950 WP_011197743.1 3218589..3222281(+) (addA) [Bacillus licheniformis strain 2709]
ATGGAGATTTCTAAACCGAAGGGCAGCACCTGGACTGATGACCAATGGAAGGCGATTGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGTTCCGGAAAAACGGCCGTGCTGGTTGAACGGATCATTCGGAAAATAACGGATCAGGAACGAC
CGGTAGATGTCGACAGGCTGCTCGTCGTCACCTTTACAAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCCGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCATTCATTCTGTCTTCAAGTCATCCGCAAATATTACTATTTGATCGATGTCGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTCGGCGATGAGGTGCTGGATGAACTGTTTGAAGAAGAATACAAAAAAGGCAATCCTGCC
TTTTTTGAACTTGTAGACAGATATACGACAGACCGTCATGACCTCGACCTGCAGCATCTTGTCAAGCGGGTCTACGAATT
TTCCCGTTCGCATCCCGATCCGGAGGGCTGGCTTCACTCATTGGCTGAACTGTATGACGCGGCTTCGGATACGAAGGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTCGTTCTGGAAGGTATGCGGCAAAAGCTGACGCGCGCG
CTTGATTTGACAAAGCAGCCCGGGGGGCCTGCTCCGCGCGCCGAAAATTTTCTCGATGATTTAGCGCAGATCGACAGGCT
GATTCAGCAGCAGGATGATTTTTCGGCCCTGCATGAGCTTGTACCGACCGTTTCGTTTCAACGATTGAAGCCATGCAAAG
GTGATGAGTACGATCCCAGATTGGTTGATGAGGCGGCAGACCTCCGCAACAGTGCGAAAAAGCAGCTTGAAAAGCTGAAA
AGCGATTATTTTTCGCGAACTCCGGAGCAGCACCTTGAGAGCCTGAGGGAAATGAAGCCTGTCATTCAGACGCTTGTCCA
GCTCGTGCTGGAGTATGGCAGGCGGTTTGCGGAAGCGAAAAAGGAAAAGGCGATCGTCGATTTTTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGTGAAAAATCCGGCGGGCGAGGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCACGAAGTGCTTGTTGATGAATACCAGGATACGAACCTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCTGAGCCGCTTTTGTTCC
TCTCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAGAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAACAGCTCATGGGAGAAAAAGTCGGGGAAGTCGAGTATGACGAACA
AGCCGAATTGAAGCTCGGCGCCTCTTATCCGCCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAACCCCGAGCG
GCGCGCCGGGTGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGGCAGATCCGCAGGCTG
ATTACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGAAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCTCAAATTATGGACGAGTTTAAACAGCAGGGAATACCCGTATACGCGAACCTCTCAACTG
GTTATTTTGAGGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCCTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTGGGTCTTGATGAGAATGAGCTTTCCTTGATCAGAATCAAAGATAAAAAAGCGCC
GTTTTACGAAGCCATGAAAGCGTACCTTGCTGCAGCAGACGGCGATGAGAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
GTCTGCTCAAGAAGTGGAGAGCTTATGCGAAAAACCACTCTGTAGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCGAGATC
CTATGAAGCGACATCATTCCGCGGCTTGTTCCGCTTTTTAAGATTTATTGAGCGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCAAGGGCTCTAAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAATCTTATCTGCTGGATAAAGAGCTCGG
TTTTGGAACGAAGTTTATCCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTTATCGCGATGAAGAAAAAACTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCCCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCGAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGACACAAGCGGCCGGGTCCGAGTGGCTGCTCCCGGATTATGA
ACGGTTTCAGGCAAAATCATATCTGGATTTCATCGGACCTGCTTTGATGAGGCACAGAGATATGGATGAATCCGGGGCGC
CGCCTGTGATTGATGAGATCCGCGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCCGCCGCGGATCTACAAGCTGAG
GCTGTCGATCAGGCCGGTGAAGAAAGACACGACCGGCTCGTCCAAATTCAAATGGGCCATGCGATTGAAGGCGCCTTTGA
ATATGAGCAGCAAGTCAGAGAACGACTGGCATGGTCATATCCATACAAGGACGCCGCCAAAGTCAGAACAAAACAATCTG
TCTCAGAAATGAAACGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCGCTTATTCGTCCATCACAGGAGGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCAGCCGAACGGGGGACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTACGCCTGAAAAGAATGAGCTCAGCCGCCTTCTAGACAGGCTTGTTGAGAAAGAGCTGTTAA
CAGAAGACCAGCGGGCAGCGATTGAAGAAGACGACATTCTTGCATTCTTTGATACGGAAATCGGACAAAAGCTTTTCGGC
GCCCGCCGCGTCGAACGGGAGGTTCCCTTTAACATGACGCTGTCCGCTAAAGAGGTTTATCCGGATCTTGAGTCAGCCGA
TGAACCTGTTTTGATACAGGGAATTATCGACTGCCTATTCGAGACGGAGGACGGTTTTTACCTTCTAGATTACAAAACCG
ACCGGATACACGGAAAGTACCGGAACGGCTTCGAAGGGGCCGAGCCGATTTTAAGAAAACGTTACGAAACACAAATTCAG
CTTTACGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGCGCGCTGTACTTTTTTGACGGAGGACACGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

3D structure

• AlphaFold DB: Q65LJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.707	100	0.717