Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K8Z23_RS25410 Genome accession   NZ_CP083129
Coordinates   4915350..4916726 (-) Length   458 a.a.
NCBI ID   WP_001085199.1    Uniprot ID   A0A9W5QZX4
Organism   Bacillus thuringiensis strain NB-176     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4910350..4921726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8Z23_RS25390 (K8Z23_25345) ispF 4911717..4912193 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  K8Z23_RS25395 (K8Z23_25350) ispD 4912310..4912990 (-) 681 WP_000288312.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  K8Z23_RS25400 (K8Z23_25355) - 4913007..4914110 (-) 1104 WP_000919681.1 PIN/TRAM domain-containing protein -
  K8Z23_RS25405 (K8Z23_25360) disA 4914273..4915346 (-) 1074 WP_000392164.1 DNA integrity scanning diadenylate cyclase DisA -
  K8Z23_RS25410 (K8Z23_25365) radA 4915350..4916726 (-) 1377 WP_001085199.1 DNA repair protein RadA Machinery gene
  K8Z23_RS25415 (K8Z23_25370) clpC 4916822..4919257 (-) 2436 WP_000971173.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  K8Z23_RS25420 (K8Z23_25375) - 4919280..4920344 (-) 1065 WP_000050837.1 protein arginine kinase -
  K8Z23_RS25425 (K8Z23_25380) - 4920349..4920897 (-) 549 WP_000128376.1 UvrB/UvrC motif-containing protein -
  K8Z23_RS25430 (K8Z23_25385) ctsR 4921068..4921529 (-) 462 WP_001244560.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49899.60 Da        Isoelectric Point: 6.9053

>NTDB_id=604751 K8Z23_RS25410 WP_001085199.1 4915350..4916726(-) (radA) [Bacillus thuringiensis strain NB-176]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETEFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=604751 K8Z23_RS25410 WP_001085199.1 4915350..4916726(-) (radA) [Bacillus thuringiensis strain NB-176]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGTAAATGTCCTGGATG
TGGTCAATGGAATACACTTGTTGAAGAAATGGAGCCAGTTGTATCATCTAGGCGCCTTAATTACGCGAATGCAATTCAAA
CGGAAGTAACAAAACCAAGACGTCTTACCGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACGGAATTTCAAGAGTTT
AATCGTGTGCTTGGTGGTGGAATTGTAGATGGGTCCTTAGTGCTTATTGGTGGAGACCCTGGGATTGGAAAATCAACATT
ACTATTACAGATTTCATCACAATTAGCAGATTCTTCATATGATGTATTATACATATCGGGTGAGGAGTCGGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGCAATCTATTTGTTGTAGCAGAGACAGACCTGCAGCGTATTGCA
GCACATATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
ACCAGGAAGTGTGGCACAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACAAAAGGAATCCCTATTTTCA
TTGTCGGGCATGTGACAAAAGAAGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACGTATCGTATTTTACGGGCTGTGAAGAACCGTTTTGGTTCCACGAATGAAATGGGTATCTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTTTTAAATCCTTCTGAAATCTTCCTTGAGGAAAGGCCGGTTGGTGTTGCAG
GATCAACAGTAGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTTTCGCTTATTATGGCAGTATTAGAAAAAAGAACAGG
TTTACTATTGCAAAATCAAGATGCATATTTAAAAGTAGCTGGTGGTTTGAAATTAGACGAACCGGCAATTGATTTAGCAG
TGGCTTTAAGTATAGCCTCAAGTTTTAGGGATAAATCTACGGCACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGTGTTATTATTCC
TAGAAAAAACTTAGGAGGATGGACAATTCCGGATGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus pneumoniae Rx1

64.238

98.908

0.635
  radA Streptococcus pneumoniae D39

64.238

98.908

0.635
  radA Streptococcus pneumoniae R6

64.238

98.908

0.635
  radA Streptococcus pneumoniae TIGR4

64.238

98.908

0.635
  radA Streptococcus mitis SK321

64.238

98.908

0.635
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633