Detailed information NTG0604747

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: K8Z23_RS20675
• Genome accession: NZ_CP083129
• Coordinates: 4012424..4016149 (-)
• Length: 1241 a.a.
• NCBI ID: WP_016077977.1
• Uniprot ID: A0A9X6Z472
• Organism: Bacillus thuringiensis strain NB-176
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4007424..4021149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8Z23_RS20625 (K8Z23_20595)	-	4008259..4008543 (+)	285	WP_016077976.1	hypothetical protein	-
K8Z23_RS20630 (K8Z23_20600)	-	4008579..4009478 (-)	900	WP_001213078.1	fumarylacetoacetate hydrolase family protein	-
K8Z23_RS20635 (K8Z23_20605)	-	4009725..4009904 (+)	180	WP_000462848.1	aspartyl-phosphate phosphatase Spo0E family protein	-
K8Z23_RS20640 (K8Z23_20610)	gerPA	4010001..4010222 (+)	222	WP_001111188.1	spore germination protein GerPA	-
K8Z23_RS20645 (K8Z23_20615)	gerPB	4010237..4010443 (+)	207	WP_001012509.1	spore germination protein GerPB	-
K8Z23_RS20650 (K8Z23_20620)	gerPC	4010511..4011125 (+)	615	WP_001070761.1	spore germination protein GerPC	-
K8Z23_RS20655 (K8Z23_20625)	gerPD	4011132..4011326 (+)	195	WP_001052807.1	spore germination protein GerPD	-
K8Z23_RS20660 (K8Z23_20630)	-	4011342..4011728 (+)	387	WP_000902327.1	spore germination protein GerPE	-
K8Z23_RS20665 (K8Z23_20635)	gerPF	4011771..4011986 (+)	216	WP_001141566.1	spore germination protein GerPF	-
K8Z23_RS20670 (K8Z23_20640)	-	4012124..4012411 (-)	288	WP_000255718.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
K8Z23_RS20675 (K8Z23_20645)	addA	4012424..4016149 (-)	3726	WP_016077977.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
K8Z23_RS20680 (K8Z23_20650)	addB	4016146..4019661 (-)	3516	WP_016077978.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
K8Z23_RS20685 (K8Z23_20655)	lepB	4019777..4020340 (-)	564	WP_000751896.1	signal peptidase I	-
K8Z23_RS20690 (K8Z23_20660)	-	4020397..4020990 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142800.96 Da
• Isoelectric Point: 4.7855

>NTDB_id=604747 K8Z23_RS20675 WP_016077977.1 4012424..4016149(-) (addA) [Bacillus thuringiensis strain NB-176]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFTEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTNWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSYRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=604747 K8Z23_RS20675 WP_016077977.1 4012424..4016149(-) (addA) [Bacillus thuringiensis strain NB-176]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAGCATGTAAGAAAGCAACTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTGGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCATTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTACTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCTG
AACCAGGACTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTCATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGATGCTGACGCTGAATTAAAACTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAATTACTCTGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCAACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCAATCGTTGGATTAAACGATGAAGACCTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAGGAACAAGAAC
TTCATGAAAAACTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGCGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATCATGACGATC
CATAAAAGTAAAGGGCTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGCCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTCCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATACACGACATTAT
CACAGCTTGCGATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCGGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACGAACTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCAACATCTTATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTGCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GATTTAGGTAAAAGGGTACTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATAGATATAAAGTACAGCTTTCGTTATATGCAAAAGCGCTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534